Separating Reflective and Fluorescent Components Using High Frequency Illumination in the Spectral Domain

Area of Science:

• Optics
• Spectroscopy
• Imaging Science

Background:

• Fluorescence is common in everyday materials but often overlooked in traditional hyperspectral imaging.
• Analyzing materials requires separating reflected light from fluorescent emissions.
• Existing methods often need multiple illuminants, limiting practical application.

Purpose of the Study:

• To develop an efficient method for separating reflectance and fluorescence spectra.
• To enable the estimation of fluorescence absorption spectra from emission spectra.
• To demonstrate the method's applicability under ambient light and for synthetic relighting.

Main Methods:

• Utilized two high-frequency illuminations in the spectral domain for efficient component separation.
• Developed a technique to estimate fluorescence absorption spectra based on emission spectra.
• Investigated the use of filters with standard light sources to create necessary illuminants.

Main Results:

• Successfully separated and recovered reflectance and fluorescence emission spectra from complex samples.
• Demonstrated the ability to estimate fluorescence absorption spectra.
• Validated the method's performance under ambient light conditions.

Conclusions:

• The proposed hyperspectral imaging technique effectively separates reflectance and fluorescence.
• This method offers a practical approach for analyzing materials with fluorescent properties.
• The technique has potential applications in various fields, including synthetic relighting.