Recent advances in CE mediated microanalysis for enzymatic and derivatization reactions

Area of Science:

• Analytical Chemistry
• Biochemistry
• Separation Science

Background:

• Capillary electrophoresis (CE) is a powerful separation technique.
• Microanalysis offers sensitive detection and reduced sample consumption.
• Previous reviews have established a foundation for CE mediated microanalysis.

Purpose of the Study:

• To provide an overview of recent applications and advances in CE mediated microanalysis.
• To cover scientific literature published between mid-2013 and early-2015.
• To summarize developments in in-line CE, enzyme analysis, on-capillary derivatization, and immobilized enzyme reactors (IMERs).

Main Methods:

• Literature review of scientific articles.
• Categorization of CE mediated microanalysis techniques.
• Summary of advancements in specific CE methodologies.

Main Results:

• Discussion of various in-line CE mediated microanalysis techniques.
• Overview of relevant papers focusing on in-line CE for enzyme analysis.
• Summarization of recent progress in on-capillary derivatization and IMERs.

Conclusions:

• CE mediated microanalysis continues to evolve with significant advancements.
• On-capillary derivatization and IMERs enhance the capabilities of CE.
• This review provides a valuable update on the field for researchers.