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Mechano growth factor-E regulates apoptosis and inflammatory responses in fibroblast-like synoviocytes of knee

Haibin Li1,2, Mingxing Lei1,2, Can Yu1,2

  • 1Key Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing, 400044, People's Republic of China.

International Orthopaedics
|September 5, 2015
PubMed
Abstract

Insights

Mechano growth factor-E (MGF-E) peptide shows therapeutic potential for osteoarthritis (OA). It regulates apoptosis and inflammation in OA fibroblast-like synoviocytes without cytotoxicity, suggesting it may aid in OA repair.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Immunology

Background:

  • Osteoarthritis (OA) is a degenerative joint disease characterized by inflammation and apoptosis in fibroblast-like synoviocytes (FLS).
  • Understanding molecular mechanisms regulating these processes is crucial for developing effective OA therapies.

Purpose of the Study:

  • To investigate the effects of mechano growth factor-E (MGF-E) peptide on apoptosis and inflammation in OA fibroblast-like synoviocytes (OA-FLS).
  • To assess the therapeutic potential of MGF-E peptide in OA.

Main Methods:

  • Cytotoxicity was evaluated using a (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) (MTS) assay.
  • Messenger RNA (mRNA) expression of lysyl oxidase (LOX) family members was analyzed via quantitative real-time polymerase chain reaction (qRT-PCR).
  • Proliferation, apoptosis, endoplasmic reticulum (ER) stress, and inflammatory cytokine levels were assessed using EdU incorporation and Western blot.

Main Results:

  • MGF-E peptide exhibited no significant cytotoxicity on OA-FLS.
  • MGF-E peptide promoted LOX expression and induced apoptosis and ER stress in OA-FLS.
  • Down-regulation of pro-inflammatory cytokines, including tumor necrosis factor alpha (TNF-α) and interleukin-1 beta (IL-1β), was observed.

Conclusions:

  • MGF-E peptide demonstrates potential anti-inflammatory properties.
  • The peptide induces apoptosis and aids in the repair of OA-FLS.
  • MGF-E peptide may hold therapeutic promise for patients suffering from osteoarthritis.
Keywords:
ApoptosisER stressFibroblast-like synoviocytesInflammatory cytokinesLysyl oxidaseMGF-E peptideOsteoarthritis

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