[Cell line of hybridoma secreting monoclonal antibodies against Echinococcus granulosus antigens]

In order to produce specific monoclonal antibodies for immunodiagnosis and immunoprophylaxis in hydatid disease, a monoclonal antibody cell line NIA2 secreting specific antibodies was established, through fusion of myeloma cells SP2/0 and spleen cells of BALB/c mice immunized with hydatid fluid from a local (Ningxia) patient and repeated screenings and clonings. The specificity was assayed via ELISA, immunoprecipitating and ultramicroscopy. Antibody titer of 1:2,560 was obtained in the supernatant of the culture medium by ELISA and a high titer of 1:163,840 in seeded ascitic fluid of mice of the same isolate. Immunoglobulin subclass of the NIA2 secreting antibody was determined to be IgG1 which showed no cross reaction to Cysticercus cellulosae. Cloned cell lines from the hybridomas were found consistent in secreting anti-Echinococcus granulosus antibody through serial passage in tissue culture for ten months.