Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 4, 2026

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

10.5K

Three-dimensional printed millifluidic devices for zebrafish embryo tests.

Feng Zhu1, Joanna Skommer1, Niall P Macdonald2

  • 1The BioMEMS Research Group, School of Applied Sciences, RMIT University , Melbourne, Victoria 3083, Australia.

Biomicrofluidics
|September 5, 2015
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rapid Fabrication of Highly Customizable Test Chambers for Behavioral Experiments with Small Aquatic Model Organisms.

Zebrafish·2026
Same author

Behavioural phenotyping with small aquatic model organisms: why digital video resolution and bitrate matter.

Behavioural pharmacology·2026
Same author

Modulation of CREB signaling contributes to anxiety-like phenotypes in a kindling model of adult zebrafish.

Experimental neurology·2026
Same author

(<i>E</i>)-4-(4-Acrylamidophenoxy)-<i>N</i>-Methylpicolinamides as b-Raf/VEGFR-2 Inhibitors with Antiangiogenic Activity in HUVEC and Zebrafish Model.

Molecules (Basel, Switzerland)·2026
Same author

Thermotactic Decision-Making in Aquatic Invertebrates: High-Resolution Behavioral Analysis of Ecotoxicological Effects.

Environmental science & technology·2026
Same author

Organic pollutants leaching from tire waste: Ecotoxicity implications for aquatic species.

Aquatic toxicology (Amsterdam, Netherlands)·2025

3D printing technologies like Stereolithography (SLA) offer rapid, cost-effective fabrication of millifluidic devices for biological research. However, careful evaluation of polymer biotoxicity is crucial for safe use with model organisms.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • 3D Printing

Background:

  • Lab-on-a-Chip (LOC) devices are valuable for in-situ analysis of small model organisms.
  • Traditional microfabrication methods are expensive and require specialized facilities, creating a barrier for many researchers.
  • There is a need for accessible, user-friendly methods to produce custom microfluidic and millifluidic devices.

Purpose of the Study:

  • To evaluate commercially available Multi-Jet Modelling (MJM) and Stereolithography (SLA) 3D printing systems for fabricating optical-grade millifluidic devices.
  • To assess the feasibility of using these 3D printing technologies to bridge the gap between bioengineering workshops and laboratory benches.
  • To provide a pathway for rapid, inexpensive, and customized device production for studies on small model organisms.

More Related Videos

Low-cost Polyethylene Terephthalate Lamination Microfluidics Designs for Multiplexed Zebrafish Imaging
05:48

Low-cost Polyethylene Terephthalate Lamination Microfluidics Designs for Multiplexed Zebrafish Imaging

Published on: September 27, 2024

798
A 3-dimensional 3D-printed Template for High Throughput Zebrafish Embryo Arraying
04:52

A 3-dimensional 3D-printed Template for High Throughput Zebrafish Embryo Arraying

Published on: June 1, 2018

7.5K

Related Experiment Videos

Last Updated: Apr 4, 2026

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

10.5K
Low-cost Polyethylene Terephthalate Lamination Microfluidics Designs for Multiplexed Zebrafish Imaging
05:48

Low-cost Polyethylene Terephthalate Lamination Microfluidics Designs for Multiplexed Zebrafish Imaging

Published on: September 27, 2024

798
A 3-dimensional 3D-printed Template for High Throughput Zebrafish Embryo Arraying
04:52

A 3-dimensional 3D-printed Template for High Throughput Zebrafish Embryo Arraying

Published on: June 1, 2018

7.5K

Main Methods:

  • Commercially available MJM and SLA 3D printers were used for device fabrication.
  • Devices were designed for culture and biotests on millimetre-sized specimens, such as zebrafish embryos.
  • Fabrication capabilities were compared against soft lithography and infrared laser micromachining.

Main Results:

  • Selected SLA technologies demonstrated user-friendly, rapid prototype production with superior feature reproduction and optical transparency.
  • MJM and SLA offer a viable alternative to traditional microfabrication for producing millifluidic devices.
  • A significant concern was identified: most tested MJM and SLA resins exhibited biotoxicity, causing developmental abnormalities in zebrafish embryos.

Conclusions:

  • Stereolithography (SLA) is a promising technology for the rapid and accurate production of millifluidic devices for biomedical research.
  • Despite the fabrication advantages, the biotoxicity of 3D printing polymers must be rigorously assessed before application in biological studies.
  • Further research is needed to develop biocompatible resins for 3D printed microfluidic devices.