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Updated: Apr 4, 2026

Microfluidics in Assessing Platelet Function
Published on: November 8, 2024
Antithrombotic activity of Vitis labrusca extract on rat platelet aggregation
Se-Uk Kwon1, Hoon-Yeon Lee, Mingjie Xin
1aDepartment of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute bDepartment of Food Industry Convergence, Wonkwang University, Iksan cHanpoong Pharm & Foods Co., Ltd. Guretdeul3-gil, Deokjin-gu dDepartment of Immunology and Institute of Medical Science, Jeonbuk National University Medical School, Jeonju, Jeonbuk, Korea.
Abstract:
Vitis labrusca is a grapevine that has antioxidant, neuroprotective, hepatoprotective, and anticarcinogenic activity. However, the antithrombotic effect of Vitis labrusca leaves on platelets is yet to be ascertained. We investigated the inhibitory effect of V. labrusca leaf extract (VLE) on platelet aggregation in vitro and ex vivo. The thromboxane B2 (TXB2) and serotonin concentrations were measured by ELISA. The flavonoids content was measured by ultraperformance liquid chromatography (UPLC). The antithrombotic activity of VLE was evaluated using various agonists in vitro. VLE strongly inhibited adenosine diphosphate (ADP)-induced platelet aggregation. In rats, VLE treatment (100 mg/kg) reduced ADP-stimulated platelet aggregation, without affecting tail bleeding and coagulation time. Moreover, VLE significantly suppressed TXB2 and serotonin secretion. UPLC analysis indicated that VLE contains quercetin, isorhamnetin, and rutin. Our results indicate that VLE possesses antiplatelet activity via the suppression of TXB2 and serotonin, without affecting bleeding. Further, we identified the flavonoids present in VLE. Thus, VLE may be a potential agent for the prevention of cardiovascular diseases.
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