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Human Herpesvirus-6 U14 Induces Cell-Cycle Arrest in G2/M Phase by Associating with a Cellular Protein, EDD

Junko Mori1, Akiko Kawabata1, Huamin Tang2

  • 1Division of Clinical Virology, Kobe University Graduate School of Medicine, Kobe, 6500017, Japan.

Plos One
|September 5, 2015
PubMed

Insights

Human herpesvirus-6 (HHV-6) infection causes cell-cycle arrest. The HHV-6 U14 protein interacts with cellular EDD protein, leading to G2/M phase arrest, crucial for viral replication.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Human herpesvirus-6 (HHV-6) is known to induce cell-cycle arrest.
  • Understanding the molecular mechanisms behind HHV-6-induced cell-cycle regulation is crucial for comprehending viral pathogenesis.

Purpose of the Study:

  • To investigate the role of the HHV-6-encoded U14 protein in inducing cell-cycle arrest.
  • To elucidate the interaction between HHV-6 U14 protein and cellular factors involved in cell-cycle regulation.

Main Methods:

  • Cell-cycle analysis of HHV-6 infected cells.
  • Co-immunoprecipitation assays to detect protein-protein interactions.
  • Analysis of U14 protein mutants to identify critical domains for EDD interaction and cell-cycle arrest.

Main Results:

  • The HHV-6 U14 protein induces cell-cycle arrest at the G2/M phase.
  • U14 protein associates with the cellular DNA-damage mediator EDD.
  • The C-terminal region of U14 is essential for its association with EDD and subsequent G2/M phase arrest.

Conclusions:

  • HHV-6 U14 protein acts as a G2/M checkpoint regulator.
  • The interaction between U14 and EDD is critical for HHV-6-mediated cell-cycle arrest.

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