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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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Sampling Methods: Sample Types01:18

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Sampling materials are classified into three main types: solid, liquid, and gas.
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DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Sample Preparation for Analysis: Overview01:21

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Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
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Systematic Sampling Method01:17

Systematic Sampling Method

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Sampling is a technique to select a portion (or subset) of the larger population and study that portion (the sample) to gain information about the population. Data are the result of sampling from a population. The sampling method ensures that samples are drawn without bias and accurately represent the population. Because measuring the entire population in a study is not practical, researchers use samples to represent the population of interest.
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High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
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Online and automated sample extraction.

Dong Wei1, Ming Li1, Kristopher W King2

  • 1Translational Sciences, Biogen Idec Inc., Cambridge, MA, USA.

Bioanalysis
|September 5, 2015
PubMed
Summary
This summary is machine-generated.

Automated sample extraction methods, including online solid-phase extraction and offline robotic platforms, enhance throughput and quality for LC-MS/MS analysis. This review covers current techniques and future trends in sample preparation automation.

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Area of Science:

  • Analytical Chemistry
  • Biotechnology
  • Pharmacology

Background:

  • Online and automated sample extraction are crucial for improving the efficiency and reliability of Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) analyses.
  • High-throughput analysis demands robust and automated sample preparation techniques to manage large sample volumes and complex matrices.
  • Advancements in automation are key to reducing human error and increasing reproducibility in analytical workflows.

Purpose of the Study:

  • To provide a comprehensive review of current online and offline automated sample extraction methodologies used in LC-MS/MS.
  • To discuss the various techniques, including online solid-phase extraction (SPE), turbulent flow chromatography, online DBS extraction, and online immunoaffinity extraction.
  • To explore the landscape of offline automated sample extraction platforms and integrated systems, alongside recent developments and future trends.

Main Methods:

  • Review of established online sample extraction techniques: online SPE, turbulent flow chromatography, online DBS extraction, and online immunoaffinity extraction.
  • Examination of offline automated sample extraction platforms, including custom robot scripts for assay automation and integrated multiple sample extraction.
  • Discussion of recent technological advancements and emerging trends in automated sample preparation for LC-MS/MS.

Main Results:

  • Online SPE, turbulent flow chromatography, online DBS extraction, and online immunoaffinity extraction are prominent automated methods for sample extraction.
  • Offline automation platforms offer flexibility through custom robot scripts for specific steps or entire assays, enabling integrated multiple sample extraction.
  • The field is characterized by continuous innovation, focusing on miniaturization, increased efficiency, and broader applicability of automated extraction techniques.

Conclusions:

  • Automated sample extraction, both online and offline, significantly boosts throughput and quality in LC-MS/MS analysis.
  • The reviewed methodologies offer diverse solutions for sample preparation challenges, catering to various analytical needs.
  • Future trends point towards more integrated, intelligent, and versatile automated systems for advanced analytical applications.