Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

4.2K
Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
4.2K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.8K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.8K
Supercritical Fluid Chromatography01:18

Supercritical Fluid Chromatography

1.3K
Supercritical fluid chromatography (SFC) provides a beneficial substitute for gas chromatography (GC) and liquid chromatography (LC) for certain samples because it merges the top attributes of both techniques. SFC allows the separation and analysis of compounds that GC or LC does not easily manage. These compounds are traditionally nonvolatile or thermally unstable, making GC unsuitable and lacking functional groups required for HPLC analysis.
SFC utilizes a supercritical fluid mobile phase,...
1.3K
Size-Exclusion Chromatography01:08

Size-Exclusion Chromatography

2.7K
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
2.7K
High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

2.0K
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmaceutical drug monitoring: the role of oral fluid sampling.

Bioanalysis·2026
Same author

Tributyltin-dysregulated periprostatic adipose tissue enhances prostate cell survival and migration, implicating the C-C motif chemokine receptor 3.

Journal of environmental sciences (China)·2026
Same author

Chemical Characterisation of <i>Cytisus striatus</i>: A Multi-Technique Approach Using GC-MS, LC-HRMS/MS, NIR, and FT-RAMAN.

Plants (Basel, Switzerland)·2026
Same author

The Potential of Volatilomics as Female Fertilization Biomarkers in Assisted Reproductive Techniques.

Biomedicines·2026
Same author

Cannabinoid Therapies in Less-Common Disorders: Clinical Evidence and Formulation Strategies.

Diseases (Basel, Switzerland)·2026
Same author

Clinical practice guideline implementation: challenges and solutions for improved cardiovascular care: A Report from the European Society of Cardiology Cardiovascular RoundTable.

European heart journal. Quality of care & clinical outcomes·2026
Same journal

A simple, sensitive microsample LC-MS assay for quercetin and isorhamnetin in mouse and human plasma: application to EMIQ treatment in myotonic dystrophy type 1.

Bioanalysis·2026
Same journal

ADA assays for high-dose biologics: redefining drug tolerance through clinical insights.

Bioanalysis·2026
Same journal

Comparison of SERS spectral data sets of blood serum samples of hypopharyngeal cancer using silver and gold nanoparticles as substrates.

Bioanalysis·2026
Same journal

The Gyrolab platform for immunogenicity assessment and biotherapeutic and biomarker analysis: technical advances and bioanalytical applications.

Bioanalysis·2026
Same journal

Simultaneous quantification of D-penicillamine, D-penicillamine disulfide, and L-cysteine-D-penicillamine disulfide in human plasma: optimization of sample preparation and mass spectrometry procedures to support bioequivalence studies.

Bioanalysis·2026
Same journal

Development and preliminary clinical application of a time-resolved fluoroimmunoassay for anti-rituximab antibodies in membranous nephropathy.

Bioanalysis·2026
See all related articles

Related Experiment Video

Updated: Apr 4, 2026

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
05:31

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

11.0K

Variations in headspace microextraction procedures and current applications in bioanalysis.

Alexandra Gonçalves1, Eugenia Gallardo1, Mário Barroso2

  • 1CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av Infante D Henrique, 6201-556 Covilhã, Portugal.

Bioanalysis
|September 5, 2015
PubMed
Summary
This summary is machine-generated.

Headspace microextraction offers an eco-friendly alternative to traditional methods, reducing solvent waste and minimizing interferences for accurate analyte analysis. This technique enhances analytical instrumentation longevity and efficiency.

More Related Videos

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.7K
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
05:29

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Published on: June 9, 2021

4.7K

Related Experiment Videos

Last Updated: Apr 4, 2026

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool
05:31

Gas Chromatography-Mass Spectrometry Paired with Total Vaporization Solid-Phase Microextraction as a Forensic Tool

Published on: May 25, 2021

11.0K
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.7K
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
05:29

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Published on: June 9, 2021

4.7K

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Chemical Engineering

Background:

  • Traditional analyte extraction methods often require large volumes of organic solvents, posing disposal challenges and environmental concerns.
  • Existing procedures can suffer from significant interferences, complicating analyte identification and accurate quantitation.
  • These drawbacks necessitate the development of more efficient and sustainable sample preparation techniques.

Purpose of the Study:

  • To introduce and evaluate headspace microextraction as a superior alternative to conventional sample preparation methods.
  • To address the limitations of traditional techniques, including solvent waste and analytical interferences.
  • To highlight the benefits of headspace microextraction for improved analyte analysis and instrumentation performance.

Main Methods:

  • Utilized microextraction techniques focusing on the headspace (vapor phase) above a sample.
  • Reduced the volume of solvents and samples required for analysis.
  • Minimized direct contact with the sample matrix to reduce interferences.

Main Results:

  • Significantly decreased the consumption and disposal of organic solvents.
  • Minimized the presence of interfering substances in the analytical process.
  • Demonstrated a reduction in sample and solvent volumes compared to traditional methods.

Conclusions:

  • Headspace microextraction presents a promising solution for overcoming the limitations of traditional sample preparation.
  • The technique offers environmental benefits through reduced solvent usage and improved analytical efficiency.
  • This method enhances the longevity and reliability of analytical instrumentation by minimizing interferences.