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A negative loop within the nuclear pore complex controls global chromatin organization.

Manuel Breuer1, Hiroyuki Ohkura1

  • 1The Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom.

Genes & Development
|September 6, 2015
PubMed
Summary

A novel negative regulatory loop within the nuclear pore complex (NPC) controls chromatin tethering. This system, involving Nup155, Nup93, and Nup62, is vital for proper genome organization.

Keywords:
Drosophilachromatinkaryosomemeiosisnuclear pore

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • The nuclear pore complex (NPC) plays a role in gene regulation by tethering chromatin.
  • Mechanisms regulating NPC-mediated chromatin tethering to prevent excessive genome attachment are not well understood.

Purpose of the Study:

  • To investigate a negative regulatory loop within the NPC that controls the chromatin attachment state.
  • To elucidate the roles of specific nucleoporins (Nup155, Nup93, Nup62) in this regulatory process.

Main Methods:

  • Depletion of Nup62 in female germline and somatic cells.
  • Co-depletion of Nup155 and Nup62.
  • Analysis of chromatin distribution in the nucleus.

Main Results:

  • Depletion of Nup62 significantly disrupts chromatin distribution.
  • The disruption caused by Nup62 depletion can be reversed by simultaneously depleting Nup155.
  • Nup155 and Nup93 recruit Nup62 to inhibit Nup155-mediated chromatin tethering.

Conclusions:

  • A negative regulatory loop involving Nup155, Nup93, and Nup62 within the NPC suppresses excessive chromatin tethering.
  • This regulatory system is essential for maintaining large-scale chromatin organization in the nucleus.