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An improved Tn7-lux reporter for broad host range, chromosomally-integrated promoter fusions in Gram-negative
Angela Glassing1, Thomas A Lewis1
1Department of Biological and Physical Sciences, Montana State University Billings, Billings, MT 59101, United States.
Journal of Microbiological Methods
|September 6, 2015
Abstract:
An improved vector for chromosomally-integrated promoter-lux fusions is described. The modified vector was tested in parallel with the unmodified vector using the well-characterized Escherichia coli araBAD promoter in the Pseudomonas aeruginosa attTn7 site. The modified mini-Tn7 showed reduced background luminescence, and increased luminescence upon induction, giving >16-fold higher induction ratio.

