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A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

Cell-free DNA: Preanalytical variables.

Abel Jacobus Bronkhorst¹, Janine Aucamp¹, Piet J Pretorius¹

¹Centre for Human Metabonomics, Biochemistry Division, North-West University, Potchefstroom 2520, South Africa.

Clinica Chimica Acta; International Journal of Clinical Chemistry

|September 6, 2015

Summary

This summary is machine-generated.

Quantitative analysis of cell-free DNA (cfDNA) is crucial for clinical applications. Standardizing preanalytic steps is essential for accurate cfDNA kinetic assessments, moving beyond single measurements.

Keywords:

Cancer Cell-free DNA Preanalytical factors Prenatal diagnostics Quantitative analysis

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Last Updated: Apr 4, 2026

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

A Standardized Liquid Biopsy Preanalytical Protocol for Downstream Circulating-Free DNA Applications

Published on: September 16, 2022

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients

Published on: September 9, 2020

Cell-Free DNA Integrity Analysis in Urine Samples

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Published on: January 5, 2017

Area of Science:

Biochemistry
Molecular Biology
Clinical Diagnostics

Background:

Cell-free DNA (cfDNA) in human blood shows promise for solid tumor diagnostics and prognostics.
Despite potential, cfDNA analysis translation to routine clinical practice remains limited.
Key barriers include understanding cfDNA origin/function, molecular characterization, and analytical consensus.

Purpose of the Study:

This review focuses on the analytical challenges in quantitative cfDNA analysis.
It highlights the importance of cfDNA kinetic assessment over single quantitative measurements.
The aim is to identify preanalytic variables critical for optimizing cfDNA analysis.

Main Methods:

Review of existing literature on cfDNA quantitative analysis.
Focus on preanalytic steps influencing cfDNA stability and detection.
Identification of confounding variables in sample handling and processing.

Main Results:

Single quantitative cfDNA assessments have limited clinical value.
Kinetic assessment of cfDNA is essential for robust qualitative characterization.
Preanalytic variables significantly impact the accuracy of cfDNA kinetic assessments.

Conclusions:

Process optimization and standardization of preanalytic steps are vital for accurate cfDNA quantitative analysis.
Addressing preanalytic confounding variables is crucial for establishing reliable cfDNA kinetic assessments.
Standardized quantitative analysis will facilitate the clinical translation of cfDNA biomarkers.