Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction Note: Oncogenic targeting of BRM drives malignancy through C/EBPβ-dependent induction of α5 integrin.

Oncogene·2024
Same author

α5β1-Integrin promotes tension-dependent mammary epithelial cell invasion by engaging the fibronectin synergy site.

Molecular biology of the cell·2017
Same author

Effective viscosity and dynamics of spreading epithelia: a solvable model.

Soft matter·2017
Same author

Generation of stable orthogonal gradients of chemical concentration and substrate stiffness in a microfluidic device.

Lab on a chip·2015
Same author

Human breast cancer invasion and aggression correlates with ECM stiffening and immune cell infiltration.

Integrative biology : quantitative biosciences from nano to macro·2015
Same author

Oncogenic targeting of BRM drives malignancy through C/EBPβ-dependent induction of α5 integrin.

Oncogene·2013

Related Experiment Video

Updated: Apr 4, 2026

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
08:19

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Published on: October 17, 2011

13.5K

Monitoring developmental force distributions in reconstituted embryonic epithelia.

L Przybyla1, J N Lakins1, R Sunyer2

  • 1Center for Bioengineering and Tissue Regeneration, Department of Surgery, University of California, San Francisco (UCSF), San Francisco, CA, USA.

Methods (San Diego, Calif.)
|September 7, 2015
PubMed
Summary

Researchers developed a new method to measure forces within cell collectives, like human embryonic stem cells. This technique maps cellular forces and protein localization in self-renewing colonies.

Keywords:
EpiblastHuman embryonic stem cellsMechanotransductionMonolayer stress microscopySelf-organizationTraction force

More Related Videos

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
08:23

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Published on: November 2, 2018

8.1K
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
10:04

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

8.9K

Related Experiment Videos

Last Updated: Apr 4, 2026

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo
08:19

Tracking Morphogenetic Tissue Deformations in the Early Chick Embryo

Published on: October 17, 2011

13.5K
Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo
08:23

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Published on: November 2, 2018

8.1K
Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics
10:04

Patterning the Geometry of Human Embryonic Stem Cell Colonies on Compliant Substrates to Control Tissue-Level Mechanics

Published on: September 28, 2019

8.9K

Area of Science:

  • Cell Biology
  • Biophysics
  • Tissue Engineering

Background:

  • Cellular organization and microenvironment influence cell signaling and response.
  • Mechanical forces from the cellular substrate and cell-cell interactions are critical but difficult to measure.
  • Understanding force dynamics is key to interpreting cellular processes.

Purpose of the Study:

  • To present a simple method for monitoring and analyzing forces generated by cell collectives.
  • To quantify cell-extracellular matrix (ECM) and cell-cell forces in human embryonic stem cell (hESC) colonies.
  • To correlate force distribution with protein localization within dynamic hESC systems.

Main Methods:

  • Developed a novel technique for measuring traction forces exerted by organized epithelial sheets.
  • Utilized human embryonic stem cells (hESC) cultured in large epithelial sheets.
  • Analyzed force magnitude and organization, and mapped protein localization relative to force distribution.

Main Results:

  • Successfully generated traction force data from large, organized epithelial sheets of hESCs.
  • Determined the magnitude and organization of cell-ECM and cell-cell forces within self-renewing colonies.
  • Demonstrated the method's ability to measure forces in dynamic hESC systems and map protein localization to force organization.

Conclusions:

  • The developed method provides a straightforward approach to monitor and analyze forces in cell collectives.
  • This technique allows for the quantification of forces within complex cellular structures like hESC colonies.
  • It enables the study of how mechanical forces influence cellular organization and behavior in dynamic systems.