Related Experiment Video
Updated: Apr 4, 2026
07:14
Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
4.3K
Osteoclasts in the interface with electrospun hydroxyapatite
Jenni Pasuri1, Jani Holopainen2, Hanna Kokkonen1
1Department of Anatomy and Cell Biology and MRC Oulu, P.O. Box 5000, FI-90014, University of Oulu, Finland.
Colloids and Surfaces. B, Biointerfaces
|September 7, 2015
Summary
Electrospun hydroxyapatite (HA) fibers support osteoclast formation and function without provoking an inflammatory response in macrophages. These biomimetic scaffolds show promise for tissue engineering applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- Electrospinning produces lightweight, resorbable, and bioinspired scaffolds crucial for tissue engineering.
- Hydroxyapatite (HA) fibers are being explored for their potential in regenerative medicine.
Purpose of the Study:
- To investigate the influence of electrospun HA fibers on macrophage and osteoclast behavior.
- To assess the inflammatory response of macrophages to HA fibers.
- To evaluate the ability of osteoclasts to form and resorb HA fiber scaffolds.
Main Methods:
- Culturing mouse macrophages (RAW 264.7) and human osteoclasts (hOC) on electrospun HA fibers in Matrigel.
- Analyzing cell morphology using fluorescent and scanning electron microscopy.
- Measuring pro-inflammatory cytokine (IL-6, TNF-α) secretion via ELISA.
- Assessing osteoclast resorption of HA nanofibers.
Main Results:
- Cell morphology varied significantly across HA fibers, Matrigel, and glass controls.
- Electrospun HA fibers did not induce an inflammatory response in macrophages, unlike lipopolysaccharide (LPS).
- While LPS stimulated cytokine secretion, HA fibers showed only a trend towards increased secretion after 4 days.
- Functional multinuclear osteoclasts formed on HA scaffolds and actively resorbed the nanofibers.
Conclusions:
- Electrospun HA fibers are biocompatible and do not elicit a pro-inflammatory response from macrophages.
- Osteoclasts can differentiate, fuse, and resorb HA nanofibers, indicating potential for bone regeneration.
- Osteoclast resorption interfaces can form on fibrous mineral scaffolds, not just continuous bone surfaces.
More Related Videos
05:41Rapid Mix Preparation of Bioinspired Nanoscale Hydroxyapatite for Biomedical Applications
Published on: February 23, 2017
20.1K
09:35Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
10.2K
Related Concept Videos
01:40Bone Remodeling
41.1K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
41.1K
01:31Osteoclasts in Bone Remodeling
4.8K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.8K
01:09Spongy Bone
12.1K
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
12.1K
01:18The Bone Matrix
9.0K
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in...
9.0K
01:30Bone Cells and Tissue
12.2K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
12.2K