Mind the gap; seven reasons to close fragmented genome assemblies

Area of Science:

• Genomics
• Microbial Biology

Background:

• Whole-genome sequencing has advanced filamentous microbe research.
• Next-generation sequencing (NGS) has made genomic studies accessible.
• Current fungal genome assemblies are often fragmented, missing key regions.

Purpose of the Study:

• To highlight the importance of producing gapless, finished genome assemblies.
• To underscore the benefits of complete genome data for biological research.

Main Methods:

• Discussion of the significance of contiguous genome assemblies.
• Review of how complete genomes aid in studying specific genetic elements and phenomena.

Main Results:

• Fragmented assemblies limit the study of intergenic DNA, non-coding regions, and repetitive elements.
• Complete genome assemblies are essential for understanding telomeres, centromeres, effectors, gene clusters, and epigenetic phenomena.

Conclusions:

• Gapless genome assemblies are vital for answering complex biological questions in filamentous microbes.
• High-quality genome data enhances our understanding of microbial evolution and adaptability.