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Updated: Apr 4, 2026
Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants
Published on: June 13, 2014
A novel host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei
Lei Zhang1, Suping Zhang2, Xin Jiang3
1State Key Lab of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Meilong Road, P.O.B. 311, 130, Shanghai, 200237, China.
Objectives:
To develop a host-vector system for heterologous protein co-expression and purification in the Trichoderma reesei (teleomorph Hypocrea jecorina) industrial strain RUT-C30.
Results:
The co-expression and purification system is based on: (i) an efficient and reliable selectable marker using bar (phosphinothricin acetyltransferase gene); (ii) a compact hygromycin B resistance marker; and (iii) a versatile integration plasmid for gene expression and knockout. Fluorescent protein genes were introduced into T. reesei and the corresponding proteins were purified from fermentation broth. The host-vector system was used in a proof-of-principle approach to achieve the co-expression of an alkaline endoglucanase and an alkaline cellobiohydrolase.
Conclusions:
This protocol can be used at an industrial scale to produce large amounts of proteins in T. reesei.
