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Growth and Quantification of MERS-CoV Infection
Christopher M Coleman1, Matthew B Frieman1
1University of Maryland School of Medicine, Microbiology and Immunology, Baltimore, Maryland.
Abstract:
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging highly pathogenic respiratory virus. Although MERS-CoV only emerged in 2012, we and others have developed assays to grow and quantify infectious MERS-CoV and RNA products of replication in vitro. MERS-CoV is able to infect a range of cell types, but replicates to high titers in Vero E6 cells. Protocols for the propagation and quantification of MERS-CoV are presented.
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