Related Experiment Video
Updated: Apr 4, 2026

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
Published on: July 10, 2017
Clipping of Telomere from Human Chromosomes Using a Chemistry-Based Artificial Restriction DNA Cutter
Takumi Ishizuka1, Yan Xu1, Makoto Komiyama2
1Division of Chemistry, Department of Medical Sciences, University of Miyazaki, Miyazaki, Japan.
Abstract:
The detection of individual telomere lengths of human chromosomes can provide crucial information on genome stability, cancer, and telomere-related diseases. However, current methods to measure telomere length entail shortcomings that have limited their use. Recently, we have developed a method for detection of individual telomere lengths (DITL) that uses a chemistry-based DNA-cutting approach. The most beneficial feature of the DITL approach is to cleave the sequence adjacent to the telomere followed by resolution of the telomere length at the nucleotide level of a single chromosome. In this unit, a protocol for successful detection of individual telomere lengths from individual chromosomes is described in detail.
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