Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The E525K β-Myosin Mutation Causes Hypocontractility in Cardiomyocytes Without Altering Loaded Crossbridge Cycling.

bioRxiv : the preprint server for biology·2026
Same author

Generation of WTC11 CLYBL-CAG_GCaMP8f cell line for fast and sensitive calcium tracking.

Stem cell research·2026
Same author

Purinergic signaling promotes gliomagenesis through nuclear calcium transients.

bioRxiv : the preprint server for biology·2026
Same author

Suspended Tissue Engineering with Assemblable Microfluidics (STEAM).

bioRxiv : the preprint server for biology·2025
Same author

Preventing hypocontractility-induced fibroblast expansion alleviates dilated cardiomyopathy.

Science (New York, N.Y.)·2025
Same author

Bioengineered Human Cardiac Ventricular Model with Transmural Helical Remodeling.

Advanced healthcare materials·2025

Related Experiment Video

Updated: Apr 4, 2026

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

3.7K

Micropost arrays for measuring stem cell-derived cardiomyocyte contractility.

Kevin M Beussman1, Marita L Rodriguez1, Andrea Leonard1

  • 1Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.

Methods (San Diego, Calif.)
|September 8, 2015
PubMed
Summary

This study details a method using micropost arrays to measure stem cell-derived cardiomyocyte contractility. This technique aids in understanding heart disease and improving cardiac cell function for therapeutic applications.

Keywords:
CardiomyocytesCell mechanicsInduced pluripotent stem cellsMicropostsSoft lithography

More Related Videos

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes
08:47

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes

Published on: December 16, 2022

2.9K
Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
06:42

Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: May 26, 2023

3.7K

Related Experiment Videos

Last Updated: Apr 4, 2026

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

3.7K
Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes
08:47

Evaluation of Cardiac Contractility Modulation Therapy in 2D Human Stem Cell-Derived Cardiomyocytes

Published on: December 16, 2022

2.9K
Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
06:42

Author Spotlight: Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: May 26, 2023

3.7K

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Stem Cell Biology

Background:

  • Stem cell-derived cardiomyocytes are crucial for studying heart disease, drug screening, and cardiac repair.
  • Assessing cardiomyocyte function is vital for understanding cardiac health and disease progression.
  • Measuring contractile force provides a direct assessment of cardiomyocyte functional capacity.

Purpose of the Study:

  • To describe a method for quantifying contractile forces generated by stem cell-derived cardiomyocytes using micropost arrays.
  • To establish a reliable assay for evaluating cardiomyocyte maturation and contractile function.
  • To provide a tool for assessing therapeutic strategies aimed at improving cardiac cell function.

Main Methods:

  • Utilized microfabrication and soft lithography to create flexible silicone micropost arrays.
  • Functionalized micropost arrays with extracellular matrix proteins for cardiomyocyte adhesion.
  • Employed live imaging and image analysis software to capture and quantify micropost deflections caused by cardiomyocyte contractions.
  • Modeled microposts as cantilever beams to calculate contractile forces.

Main Results:

  • Developed a reproducible assay to measure cardiomyocyte contractile forces.
  • Quantified contractile forces by analyzing micropost deflections using specialized image analysis code.
  • Successfully applied the assay to evaluate methods for enhancing stem cell-derived cardiomyocyte maturation and function.

Conclusions:

  • Micropost array technology offers a precise method for measuring stem cell-derived cardiomyocyte contractility.
  • This assay is valuable for fundamental research in cardiac development and disease.
  • The technique supports the development and screening of interventions to improve cardiac cell function for regenerative medicine.