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Related Experiment Video

Updated: Apr 4, 2026

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A long downstream probe-based platform for multiplex target capture.

Ping Wang1, Jianqin Huang2, Yingwu Xu2

  • 1Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Agriculture and Forestry University, Lin'an 311300, China; National Center for Protein Science Shanghai, Shanghai 201210, China.

Analytical Biochemistry
|September 8, 2015
PubMed
Summary
This summary is machine-generated.

A new detection platform uses ligase detection reaction (LDR) and polymerase chain reaction (PCR) for rapid, specific identification of multiple targets. This method achieves high sensitivity and accuracy, suitable for field and clinical surveillance.

Keywords:
Concordance ratesLigase detection reactionSingle-strand long downstream probeStuffer sequenceSuppressor

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • Multiplex target detection is crucial for various surveillance applications.
  • Existing methods may lack specificity, sensitivity, or scalability for complex sample types.

Purpose of the Study:

  • To develop a simple, rapid, and highly specific platform for multiplex target detection.
  • To enhance sensitivity and enable identification of multiple targets simultaneously.

Main Methods:

  • Generation of single-strand long downstream probes (ssLDP) integrated with ligase detection reaction (LDR).
  • Ladder-like polymerase chain reaction (PCR) amplicons with variable-length stuffer sequences for size-based identification.
  • Electrophoresis for amplicon size analysis and target identification.

Main Results:

  • The platform demonstrated high specificity and sensitivity, detecting down to 1.2 × 10^3 copies for single or mixed target plasmids.
  • Electrophoresis showed 98.73-100% concordance with microarray results for seven suppressors in 79 field samples.
  • The method proved effective in a potato virus suppressor detection model.

Conclusions:

  • The developed multiplexed diagnostic platform offers a simple, rapid, and accurate solution for target detection.
  • This strategy is highly adaptable for detecting numerous targets in field and clinical surveillance settings.
  • The technique shows significant potential for broad application in molecular diagnostics.