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Related Experiment Videos

Particles and molecules in endodontic leakage.

H W Kersten, W R Moorer

    International Endodontic Journal
    |May 1, 1989
    PubMed
    Summary

    Root canal obturation with gutta-percha effectively prevents leakage of bacteria-sized particles and large molecules only when both sealer and pressure are used. However, microleakage of small molecules like butyric acid and methylene blue persists regardless of the obturation method.

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    Area of Science:

    • Endodontics
    • Dental Materials Science
    • Microbiology

    Background:

    • Microleakage following root canal obturation is a significant concern.
    • It is hypothesized that microleakage can lead to periapical disease and impede tissue repair.

    Purpose of the Study:

    • To evaluate the effectiveness of gutta-percha obturation in preventing leakage of bacteria-sized particles and large protein molecules.
    • To compare the leakage of methylene blue dye with a small bacterial metabolic product of similar molecular size.

    Main Methods:

    • Standardized preparation and four different obturation techniques using gutta-percha on 46 human incisor root sections.
    • Coronal reservoirs were filled with latex beads, endotoxin, butyric acid, valeric acid, and methylene blue.
    • Apical samples were analyzed after 1 and 2 weeks for the presence of contaminants using microscopy, limulus lysate test, gas-chromatography, and spectrophotometry.

    Main Results:

    • Preventing leakage of bacteria-sized particles and large molecules required both sealer and pressure during gutta-percha obturation.
    • Leakage of butyric acid was comparable to methylene blue leakage.
    • Microleakage of small molecules (butyric acid, methylene blue) could not be prevented by any tested obturation method.

    Conclusions:

    • Effective prevention of microleakage by gutta-percha obturation necessitates the use of both sealer and pressure.
    • Small molecules, such as bacterial metabolic products and dyes like methylene blue, exhibit significant microleakage irrespective of obturation technique.
    • Further research is needed to address the persistent microleakage of small molecules in root canal treatments.

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