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VING: a software for visualization of deep sequencing signals.

Marc Descrimes1, Yousra Ben Zouari2, Maxime Wery3

  • 1ncRNA, Epigenetics and Genome Fluidity, Institut Curie, PSL Research University, CNRS UMR3244, Université Pierre et Marie Curie, 26 rue d'Ulm, 75248, Paris Cedex 05, France. marc.descrimes@curie.fr.

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Summary

VING is a new R script for visualizing next-generation sequencing (NGS) data coverage. It generates publication-ready figures from NGS mapping files, ideal for multiple experiments.

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Area of Science:

  • Bioinformatics
  • Genomics
  • Computational Biology

Background:

  • Next-generation sequencing (NGS) data analysis requires mapping reads to a reference genome.
  • Genome browsers are commonly used for visualization but lack versatility for publication-ready NGS data coverage, especially for multiple experiments.

Purpose of the Study:

  • To develop a tool for generating publication-quality visualizations of NGS data coverage.
  • To provide a versatile solution for representing NGS data from multiple experiments simultaneously.

Main Methods:

  • Development of 'VING', a stand-alone R script.
  • Input includes NGS mapping files and genome annotations.
  • Offers multiple viewing options, including strand-specific views and heatmap mode.

Main Results:

  • VING produces accurate snapshots of NGS coverage signals for specified genomic regions.
  • Facilitates representation of multiple experiments in a single figure using a heatmap mode.
  • Generates high-quality figures suitable for publication.

Conclusions:

  • VING is a valuable tool for NGS data representation in regions of interest.
  • The script is available at http://vm-gb.curie.fr/ving/.
  • A Galaxy wrapper is also available in the Galaxy tool shed.