Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Data Validation01:15

Data Validation

3.6K
Method validation is a crucial process in analytical chemistry designed to confirm that a given method consistently produces reliable and high-quality results. This process is essential when a method is applied to different sample matrices or when procedural modifications are made, ensuring that the results meet acceptable standards across various applications.
Key parameters for method validation include:
3.6K
Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

18.7K
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
18.7K
In Vitro Drug Release Testing: Overview, Development and Validation01:10

In Vitro Drug Release Testing: Overview, Development and Validation

537
In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...
537
Immunoprecipitation01:20

Immunoprecipitation

8.0K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
8.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neurofilament Light Chain as a Biomarker in Neurology.

European neurology·2026
Same author

MicroRNAs as Biomarkers of Cervical Cancers.

International journal of molecular sciences·2026
Same author

Selected Proteins Involved in the Neuropathology of ASD as the Candidates for Fluid Biomarkers.

Molecules (Basel, Switzerland)·2026
Same author

The Potential of A Disintegrin and Metalloproteinase (ADAM) Proteins as Clinically Relevant Biomarkers in Colorectal Cancer: A Comprehensive Analysis.

Cancers·2026
Same author

Evaluation of chemerin levels in the pathogenesis of psoriasis.

Frontiers in medicine·2026
Same author

Comprehensive first-trimester targeted metabolomics for early prediction and understanding of GDM pathophysiology.

Frontiers in molecular biosciences·2026

Related Experiment Video

Updated: Apr 4, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

11.6K

A Practical Guide to Immunoassay Method Validation.

Ulf Andreasson1, Armand Perret-Liaudet2, Linda J C van Waalwijk van Doorn3

  • 1Clinical Neurochemistry Laboratory, Institute of Neuroscience and Physiology, The Sahlgrenska Academy, University of Gothenburg , Mölndal , Sweden.

Frontiers in Neurology
|September 9, 2015
PubMed
Summary

This study provides detailed standard operating procedures (SOPs) and a validation report template for assay method validation. These resources ensure reliable measurement of biochemical markers, crucial for diagnosing diseases like Alzheimer's and developing new drugs.

Keywords:
immunoassayslimits of quantitationmethod validationprecisionrobustness

More Related Videos

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

29.0K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.9K

Related Experiment Videos

Last Updated: Apr 4, 2026

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

11.6K
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
08:05

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

29.0K
Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform
09:02

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Published on: November 10, 2016

10.9K

Area of Science:

  • Biochemistry
  • Clinical Medicine
  • Biomarker Discovery

Background:

  • Biochemical markers are vital for diagnosing and managing diseases, including neurological disorders like Alzheimer's disease.
  • Enzyme-linked immunosorbent assay (ELISA) is commonly used for measuring low-abundance biomarkers.
  • Variability in ELISA method quality can lead to systematic and random errors, necessitating rigorous assay performance control.

Purpose of the Study:

  • To address the lack of detailed experimental guidance for method validation.
  • To provide objective evidence that analytical methods meet intended use requirements.
  • To improve the reliability of biochemical marker measurements in research, clinical settings, and drug development.

Main Methods:

  • Development of standard operating procedures (SOPs) with step-by-step instructions for various validation parameters.
  • Creation of a validation report template for structured presentation of results.
  • Focus on immunochemical methods and multicenter evaluations, with general applicability to other technologies.

Main Results:

  • A comprehensive set of SOPs for method validation parameters.
  • A template for a validation report to standardize result presentation.
  • Guidelines applicable to various analytical methods beyond immunochemistry.

Conclusions:

  • The developed SOPs and template facilitate rigorous method validation.
  • Standardized validation processes enhance the reliability of biochemical marker data.
  • This work supports consistent and accurate biomarker analysis across different applications and settings.