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Assessment of MALDI-TOF MS as Alternative Tool for Streptococcus suis Identification

Marta Pérez-Sancho1, Ana Isabel Vela2, Teresa García-Seco3

  • 1Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense , Madrid , Spain ; Campus de Excelencia Internacional (CEI) Moncloa, Universidad Politécnica de Madrid (UPM), Universidad Complutense de Madrid (UCM) , Madrid , Spain.

Frontiers in Public Health
|September 9, 2015
PubMed

Insights

Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) accurately identifies Streptococcus suis. Updating the database and lowering the score threshold improved identification rates to 96.9%.

Area of Science:

  • Microbiology
  • Veterinary Diagnostics
  • Mass Spectrometry

Background:

  • Streptococcus suis is a significant zoonotic pathogen.
  • Accurate identification of S. suis is crucial for disease control in animal and human health.
  • Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a rapid identification method.

Purpose of the Study:

  • To evaluate the accuracy of MALDI-TOF MS for S. suis identification.
  • To compare MALDI-TOF MS with PCR and a commercial identification system.
  • To optimize the MALDI-TOF MS identification criteria for S. suis.

Main Methods:

  • PCR-based assay used as the gold standard for S. suis identification.
  • MALDI-TOF MS analysis of S. suis isolates from various sources.
  • Comparison of MALDI-TOF MS results with the Rapid ID 32 STREP system.
  • Database update for MALDI-TOF MS with new S. suis spectra.

Main Results:

  • Initial MALDI-TOF MS identification showed variable accuracy with standard thresholds.
  • Updating the S. suis database significantly increased mean score values.
  • A reduced threshold score (≥2.000) with the updated database correctly identified 96.9% of isolates.
  • MALDI-TOF MS demonstrated excellent performance for S. suis identification.

Conclusions:

  • MALDI-TOF MS is a reliable and efficient method for S. suis identification.
  • Optimizing the database and adjusting the score threshold enhances accuracy.
  • MALDI-TOF MS offers a valuable alternative to traditional methods for S. suis diagnostics.
Keywords:
MALDI-TOF MSPCRStreptococcus suisbiochemical testsidentification

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