Related Experiment Video
Updated: Apr 4, 2026

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
Published on: September 30, 2016
Evolutionary divergence of Ure2pA glutathione transferases in wood degrading fungi
Thomas Roret1, Anne Thuillier2, Frédérique Favier1
1Université de Lorraine, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France; CNRS, UMR 7036 CRM(2), F-54506 Vandoeuvre-lès-Nancy, France.
Abstract:
The intracellular systems of detoxification are crucial for the survival of wood degrading fungi. Within these systems, glutathione transferases could play a major role since this family of enzymes is specifically extended in lignolytic fungi. In particular the Ure2p class represents one third of the total GST number in Phanerochaete chrysosporium. These proteins have been phylogenetically split into two subclasses called Ure2pA and Ure2pB. Ure2pB can be classified as Nu GSTs because of shared structural and functional features with previously characterized bacterial isoforms. Ure2pA can rather be qualified as Nu-like GSTs since they exhibit a number of differences. Ure2pA possess a classical transferase activity, a more divergent catalytic site and a higher structural flexibility for some of them, compared to Nu GSTs. The characterization of four members of this Ure2pA subclass (PcUre2pA4, PcUre2pA5, PcUre2pA6 and PcUre2pA8) revealed specific functional and structural features, suggesting that these enzymes have rapidly evolved and differentiated, probably to adapt to the complex chemical environment associated with wood decomposition.
More Related Videos
06:58Author Spotlight: Evaluation of Entomopathogenic Fungi in Wild Monochamus alternatus Populations for Biocontrol Applications in Forest Wood Borers
Published on: September 29, 2023
07:47Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
Published on: November 6, 2014
Related Concept Videos
Evolutionary Relationships through Genome Comparisons
Microbial Phylogeny
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Fungal Group Zygomycota
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Evolution of New Traits in Microbes