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Reliability of Intra-Retinal Layer Thickness Estimates.

Timm Oberwahrenbrock¹, Maria Weinhold², Janine Mikolajczak¹

¹NeuroCure Clinical Research Center, Charité-Universitätsmedizin Berlin, Berlin, Germany.

|September 9, 2015

Summary

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Optical coherence tomography (OCT) measurements of intra-retinal layer thickness are crucial for multiple sclerosis (MS) research. This study found that averaging thickness estimates over larger areas, specifically a 6mm diameter around the fovea, demonstrates excellent repeatability for most retinal layers.

Area of Science:

Ophthalmology
Neuroscience
Medical Imaging

Background:

Optical coherence tomography (OCT) is increasingly used in multiple sclerosis (MS) research to measure intra-retinal layer thickness.
Current methods for estimating mean retinal layer thickness in MS studies vary significantly.
There is a lack of data on the reliability of different thickness estimation approaches.

Purpose of the Study:

To evaluate the repeatability of different intra-retinal layer thickness estimation methods using OCT.
To provide crucial data on the reliability of OCT-based thickness measurements for clinical MS studies.

Main Methods:

Systematic retrieval of studies using macular OCT scans for intra-retinal layer segmentation in MS patients from PubMed.
Generation of datasets with repeated OCT measurements from 15 healthy subjects and 13 MS patients using Cirrus HD-OCT and Spectralis SD-OCT devices.

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Analysis of repeatability using intra-class correlation coefficients and coefficients of repeatability for various thickness estimation approaches.

Main Results:

A review identified 27 relevant articles, with Spectralis and Cirrus HD-OCT being the most common devices.
Multiple thickness estimation methods were identified for Spectralis (10) and Cirrus OCT (2 scan settings).
Thickness estimates averaging larger areas showed excellent repeatability across most retinal layers, with the exception of the outer plexiform layer (OPL).

Conclusions:

The thickness estimate derived from a 6mm-diameter area around the fovea demonstrates good reliability and should be favored in OCT-based clinical MS research.
Assessment of the outer plexiform layer (OPL) thickness showed weak reliability and requires further investigation before it can be considered a dependable parameter in MS studies.