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Updated: Apr 4, 2026

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Oral Health Assessment by Lay Personnel for Older Adults
Published on: February 2, 2020
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Stain Removal Assessment of Two Manual Toothbrushes with an Interproximal Tooth Stain Index
Summary
Both the Oral-B Pulsar (OBP) and Colgate Whitening (CW) manual toothbrushes effectively removed tooth stain over two weeks. The new Interproximal Modified Lobene Stain Index proved reliable for assessing stain, including in hard-to-reach areas.
Area of Science:
- Dental hygiene
- Preventive dentistry
- Oral health research
Background:
- Dental stain is a common aesthetic concern.
- Accurate measurement of stain, especially in interproximal areas, is crucial for evaluating oral hygiene efficacy.
- Existing indices may not fully capture stain in hard-to-reach regions.
Purpose of the Study:
- To evaluate a novel Interproximal Modified Lobene Stain Index for measuring interproximal tooth stain.
- To compare the stain removal efficacy of two distinct manual toothbrushes: Oral-B Pulsar (OBP) and Colgate Whitening (CW).
Main Methods:
- A two-week, randomized, examiner-blind, parallel-group clinical trial was conducted.
- Participants with an average Modified Lobene Stain Index of ≥ 1.5 were enrolled.
- Stain was assessed at baseline and after two weeks using the Modified Lobene Stain Index and the new Interproximal Modified Lobene Stain Index.
Main Results:
- Both OBP and CW toothbrushes demonstrated statistically significant reductions in tooth stain from baseline.
- Median stain reductions were 78% (OBP) and 60% (CW) by Modified Lobene Stain Index.
- The Interproximal Modified Lobene Stain Index showed higher median stain removal percentages: 92% (OBP) and 83% (CW) for interproximal sites.
Conclusions:
- Both manual toothbrushes are effective for stain removal, including in interproximal areas.
- The Interproximal Modified Lobene Stain Index provides clinically relevant and consistent results compared to the traditional index.
- The new index successfully assesses stain in difficult-to-reach interproximal regions.

