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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
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Polymeric Nanocarriers for Non-Viral Gene Delivery
Journal of Biomedical Nanotechnology
|September 10, 2015
Summary
Non-viral gene delivery systems offer a safer alternative to viral vectors for treating diseases. This review highlights polymeric nanocarriers and strategies to overcome biological barriers for improved gene therapy.
Area of Science:
- Biotechnology
- Nanomedicine
- Molecular Biology
Background:
- Gene therapy shows potential for treating genetic and acquired diseases.
- Non-viral gene delivery systems are increasingly favored over viral vectors due to enhanced safety and reduced immunogenicity.
- Effective nucleic acid delivery faces significant biological barriers impacting transfection efficiency.
Purpose of the Study:
- To review biological barriers in non-viral gene delivery.
- To discuss advancements in overcoming these barriers.
- To highlight polymeric nanocarriers and strategies for improved gene transfer.
Main Methods:
- Review of current literature on non-viral gene delivery.
- Discussion of biological barriers to transfection.
- Analysis of polymeric nanocarriers: polyethylenimine (PEI), poly(L-lysine) (PLL), poly[2-(dimethylamino) ethyl methacrylate] (PDMAEMA), polyamidoamine (PAMAM), chitosan, and poly(amino-co-ester)s (PAEs).
Main Results:
- Identified key biological barriers hindering non-viral gene delivery.
- Detailed various strategies developed over two decades to overcome these obstacles.
- Highlighted the role of specific polymeric nanocarriers in enhancing gene transfer.
Conclusions:
- Polymeric nanocarriers are crucial for safe and efficient non-viral gene delivery.
- Overcoming biological barriers is key to advancing gene therapy.
- Continued research into novel vectors and strategies is essential for therapeutic success.
