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Related Experiment Videos

Increasing potentiality in N.G.F. treated PC12 cells.

S M Peirone, M R Amedeo, G Filogamo

    Archivio Italiano Di Anatomia E Di Embriologia. Italian Journal of Anatomy and Embryology
    |July 1, 1989
    PubMed
    Summary

    Neural crest-derived PC12 cells, when induced by nerve growth factor (NGF), promote the formation of myogenic cells from chick somites. These new muscle cells, in turn, induce differentiation in the PC12 cells, revealing neural crest multipotentiality.

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    Area of Science:

    • Developmental Biology
    • Cell Biology
    • Neuroscience

    Background:

    • PC12 cells are a rat pheochromocytoma cell line derived from the neural crest.
    • PC12 cells can be induced to differentiate into neuron-like cells by nerve growth factor (NGF).
    • Somites are blocks of mesoderm that give rise to skeletal muscle, vertebrae, and dermis.

    Purpose of the Study:

    • To investigate the interaction between NGF-treated PC12 cells and chick embryo somites.
    • To determine if PC12 cells influence somite differentiation.
    • To explore the potential for reciprocal induction between neuronal and myogenic cells.

    Main Methods:

    • Co-cultivation of chick somites and PC12 cells in synthetic medium with NGF.
    • Microscopic observation of cell morphology and behavior over 10 days.
    • Immunohistochemical analysis using anti-desmin antiserum.

    Main Results:

    • NGF-treated PC12 cells extended processes and interacted with somitic mesoderm.
    • Myogenic cells (mononucleated and multinucleated) formed in co-cultures, identified by desmin positivity.
    • These myogenic cells did not form in somite-only cultures.
    • Desmin positivity was also observed in some PC12 cells after 10 days of co-culture.

    Conclusions:

    • NGF-treated PC12 cells induce myogenic differentiation in chick somites.
    • Newly formed muscle cells exert an inductive effect on PC12 cells.
    • This suggests that neural crest-derived PC12 cells retain multipotentiality and can be influenced by muscle cells.

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