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Opposite Effects of Lysophosphatidylethanolamines on Conformation of OmpF-like Porin from Yersinia pseudotuberculosis

Ludmila A Davydova, Nina M Sanina1, Olga D Novikova

  • 1Department of Biochemistry, Microbiology and Biotechnology, School of Natural Sciences, Far Eastern Federal University, 690950, Vladivostok, Russia. sanina.nm@dvfu.ru.

Protein and Peptide Letters
|September 10, 2015
PubMed

Insights

Bacterial stress induces lysophosphatidylethanolamine (LPE), a lipid that alters OmpF-like porin (YOmpF) stability. Unsaturated LPE enhances YOmpF thermostability, while saturated LPE destabilizes it.

Area of Science:

  • Microbiology
  • Biochemistry
  • Structural Biology

Background:

  • Lysophosphatidylethanolamine (LPE) is a bacterial lipid formed under stress conditions.
  • LPE accumulation enhances the thermostability of Yersinia pseudotuberculosis OmpF-like porin (YOmpF).
  • The influence of LPE's fatty acid composition on YOmpF conformation was previously unclear.

Purpose of the Study:

  • To investigate the role of LPE fatty acid composition in YOmpF conformational changes.
  • To determine how saturated and unsaturated LPE affect YOmpF stability and function.

Main Methods:

  • Analysis of LPE composition in Yersinia pseudotuberculosis under phenol stress.
  • Comparative studies using unsaturated MOPE and saturated MPPE on YOmpF conformation.
  • Assessment of YOmpF thermal stability and oligomeric state.

Main Results:

  • Under stress, unsaturated LPE levels were 3.5 times higher than saturated LPE.
  • Unsaturated MOPE increased YOmpF thermal stability by promoting dense monomer packing and preserving trimeric form.
  • Saturated MPPE weakened inter-monomer contacts, leading to YOmpF dissociation at elevated temperatures.

Conclusions:

  • LPE's fatty acid composition critically influences YOmpF conformation and stability.
  • Unsaturated LPE stabilizes YOmpF, potentially affecting channel permeability under stress.
  • Saturated LPE destabilizes YOmpF, suggesting differential roles in bacterial stress response.

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