Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bacterial Phylum Actinobacteria01:30

Bacterial Phylum Actinobacteria

860
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
860
Defense Against Bacterial Pathogens01:31

Defense Against Bacterial Pathogens

3.5K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rifamycin Structural Modifications Attenuate PXR Binding and CYP3A4 Induction.

Journal of medicinal chemistry·2026
Same author

Cytochrome P450-Mediated Metabolism of Antimycobacterial <i>N</i>α-Aroyl-<i>N</i>-aryl-phenylalanine Amides.

ACS infectious diseases·2026
Same author

Loss-of-Function Mutations in the Penicillin-Binding Protein PonA1 Confer Agar-Dependent Resistance to Durlobactam in <i>Mycobacterium abscessus</i>.

Antibiotics (Basel, Switzerland)·2026
Same author

Multicentre preclinical profiling of apramycin for the treatment of nontuberculous mycobacteria.

EBioMedicine·2026
Same author

A potent phenylalkylamine disrupts mycobacterial membrane bioenergetics and augments bactericidal activity of bedaquiline.

iScience·2025
Same author

Preparation of Agar Bead Embedded Mycobacterium abscessus to Inoculate Immunocompetent Mice Intratracheally.

Journal of visualized experiments : JoVE·2025
Same journal

<i>In vitro</i> antibacterial activity of gepotidacin in combination with other antimicrobial agents against <i>Neisseria gonorrhoeae</i> isolates.

Antimicrobial agents and chemotherapy·2026
Same journal

Development of domain-specific probes of <i>Plasmodium falciparum</i> heat shock protein 70-1.

Antimicrobial agents and chemotherapy·2026
Same journal

Addressing therapeutic options for KPC-3-producing ST307-<i>Klebsiella pneumoniae</i>: insights from <i>in vitro</i> evolution and mutant prevention strategies.

Antimicrobial agents and chemotherapy·2026
Same journal

Indole-based hybrids target both asexual parasites and gametocytes of <i>Plasmodium falciparum</i> and synergize with lumefantrine.

Antimicrobial agents and chemotherapy·2026
Same journal

Lineage-specific loss of the type VI secretion system in <i>Acinetobacter baumannii</i> ST19 is associated with reduced accessory genome content.

Antimicrobial agents and chemotherapy·2026
Same journal

Genomic diversity and topical antimicrobial resistance in <i>Staphylococcus aureus</i> from clinical and carriage populations in the New York-New Jersey region.

Antimicrobial agents and chemotherapy·2026
See all related articles

Related Experiment Video

Updated: Apr 4, 2026

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

3.1K

Eagle Effect in Nonreplicating Persister Mycobacteria.

Mu-Lu Wu1, Jasmie Tan1, Thomas Dick2

  • 1Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Singapore.

Antimicrobial Agents and Chemotherapy
|September 10, 2015
PubMed
Summary
This summary is machine-generated.

Fluoroquinolone antibiotics kill nonreplicating Mycobacterium smegmatis by triggering a protein synthesis-dependent cell death pathway at low doses. This pathway is shut off at higher concentrations, revealing a unique drug response.

More Related Videos

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
09:57

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Published on: December 14, 2016

11.0K
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

1.8K

Related Experiment Videos

Last Updated: Apr 4, 2026

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli
12:29

Population and Single-Cell Analysis of Antibiotic Persistence in Escherichia coli

Published on: March 24, 2023

3.1K
Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria
09:57

Visualization of the Charcoal Agar Resazurin Assay for Semi-quantitative, Medium-throughput Enumeration of Mycobacteria

Published on: December 14, 2016

11.0K
Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
07:44

Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes

Published on: February 14, 2025

1.8K

Area of Science:

  • Microbiology
  • Pharmacology
  • Bacteriology

Background:

  • Nonreplicating bacteria, such as Mycobacterium smegmatis in a Loebel persistence model, present challenges for antibacterial drug efficacy.
  • Understanding drug activity against persistent bacterial populations is crucial for developing new treatments.

Purpose of the Study:

  • To investigate the microbicidal activities of various antibacterials against nonreplicating Mycobacterium smegmatis.
  • To elucidate the mechanism of action for fluoroquinolones exhibiting potent activity in this model.

Main Methods:

  • Culturing Mycobacterium smegmatis in a starvation-based Loebel model to induce persistence.
  • Determining the microbicidal activity of different classes of antibacterials.
  • Conducting dose-response studies and evaluating the effect of chloramphenicol pretreatment on fluoroquinolone activity.

Main Results:

  • Most tested antibacterials lost their microbicidal activity against nonreplicating M. smegmatis.
  • Fluoroquinolones retained significant lethal potency.
  • A paradoxical Eagle effect was observed, where lower fluoroquinolone concentrations were more effective than higher ones.
  • Chloramphenicol pretreatment inhibited the lethal effect of gyrase inhibitors, indicating protein synthesis dependence.

Conclusions:

  • Fluoroquinolones exhibit potent microbicidal activity against nonreplicating Mycobacterium smegmatis.
  • Low fluoroquinolone concentrations appear to induce a cell death pathway dependent on protein synthesis.
  • This cell death pathway is suppressed at higher fluoroquinolone concentrations, explaining the observed Eagle effect.