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Related Experiment Video

Updated: Apr 4, 2026

Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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Dynamin function is important for chemokine receptor-induced cell migration.

Richard O Jacques1, Shirley C Mills1, Paula Cazzonatto Zerwes1

  • 1School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, UK.

Cell Biochemistry and Function
|September 10, 2015
PubMed
Summary

Dynamin GTPase inhibition affects CC chemokine receptor (CCR) cell migration but not CXC chemokine receptor (CXCR) migration. This suggests distinct signaling pathways control CCR and CXCR cell movement, offering potential therapeutic targets.

Keywords:
chemokine receptorchemotaxisdynamininternalizationsignalling

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Chemokine receptors CCR5 and CXCR4 are crucial for HIV entry and mediate cellular responses like chemotaxis (CTX).
  • Understanding the signaling pathways governing CC and CXC chemokine receptor-mediated cell migration is essential for targeted therapeutic strategies.

Purpose of the Study:

  • To investigate the specific signaling pathways involved in CC and CXC chemokine receptor-mediated cell migration.
  • To determine the role of dynamin GTPase in these migration processes.

Main Methods:

  • Utilized THP-1 cells and activated T lymphocytes.
  • Employed dynamin inhibitors dynasore and Dyngo-4a to block dynamin I and II GTPase activity.
  • Assessed cell migration (CTX) in response to CCL3 and other CC and CXC chemokines.

Main Results:

  • Dynasore completely inhibited CCL3-stimulated CTX in THP-1 cells, while Dyngo-4a showed reduced efficacy.
  • Dynasore did not block cell migration mediated by CXCR4.
  • Blocking dynamin-driven internalization did not affect CC or CXC receptor-induced migration, suggesting dynamin's role in cytoskeletal interaction.

Conclusions:

  • A functional difference exists in the control of CC versus CXC chemokine receptor-mediated cell migration.
  • Distinct signaling networks are employed by different chemokine receptor classes.
  • These findings may lead to the identification of specific therapeutic targets to inhibit receptor-mediated cell migration.