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Published on: September 5, 2013
The Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) Genes among Clinical Isolates of
Abdolmajid Ghasemian1, Shahin Najar Peerayeh1, Bita Bakhshi1
1Dept. of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Background:
Isolates of Staphylococcus aureus express a myriad of adhesive surface proteins that play important role in colonization of the bacteria on nasal and skin surfaces, beginning the process of pathogenesis. The aim of this study was to screen several of the Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) genes among the isolate of S. aureus from hospitalized children.
Methods:
A total of 22 S. aureus isolates were collected from hospitalized children in Tehran from 2012 to 2013. Detection of the mecA and several adhesive surface proteins genes including clfA, B (encoding clumping factors A, B); fnbA, B (encoding finronectin binding proteins A, B); fib (encoding fibrinogen binding protein); eno (encoding laminin binding protein); cna (encoding collagen binding protein); ebps (encoding elastin binding protein) and bbp (encoding bone sialo-protein binding protein), was performed by PCR.
Results:
The clfAB genes were detected among all the isolates. The prevalence of fnbA, fnbB, fib, eno, cna, ebps and bbp was 63%, 6%, 50%, 59%, 82%, 63%, 9% and 0%, respectively.
Conclusion:
The high prevalence of these genes is important for future plans in vaccine designation. MRSA and MSSA isolates similarly can produce adhesive surface proteins for colonization.
Insights
Staphylococcus aureus adhesive genes like clfA and clfB are prevalent in hospitalized children, indicating their role in bacterial colonization. This finding is crucial for developing future vaccines against S. aureus infections.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
- Molecular Biology
Background:
- Staphylococcus aureus utilizes numerous adhesive surface proteins, known as Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs), for colonization.
- These MSCRAMMs are critical for initiating pathogenesis on host surfaces like nasal passages and skin.
Purpose of the Study:
- To screen for the presence of several MSCRAMM genes in Staphylococcus aureus isolates obtained from hospitalized children.
- To investigate the prevalence of specific adhesion genes associated with S. aureus colonization and pathogenesis.
Main Methods:
- Collected 22 S. aureus isolates from hospitalized children in Tehran between 2012 and 2013.
- Employed Polymerase Chain Reaction (PCR) to detect the mecA gene and various MSCRAMM genes, including clfA, clfB, fnbA, fnbB, fib, eno, cna, ebps, and bbp.
Main Results:
- The clfA and clfB genes were universally detected in all 22 S. aureus isolates.
- Prevalence rates for other screened genes were: fnbA (63%), fnbB (6%), fib (50%), eno (59%), cna (82%), ebps (63%), and bbp (0%).
Conclusions:
- The high prevalence of these adhesive protein genes underscores their significance in S. aureus colonization.
- Findings support the potential for vaccine development targeting these MSCRAMM genes.
- Both Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Susceptible Staphylococcus aureus (MSSA) isolates possess adhesive surface proteins essential for colonization.
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