Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recombinant DNA01:09

Recombinant DNA

104.9K
Overview
104.9K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

15.0K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
15.0K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

7.4K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reconstructing enzyme evolution by protein engineering.

FEBS letters·2026
Same author

The Discovery of <i>N</i> <sup>2</sup>,<i>N</i> <sup>2</sup>‑Dimethylguanine Hydrolases Unravels General Molecular Principles of Enzyme Evolvability and Promiscuity.

ACS catalysis·2026
Same author

On the Origin of Substrate Specificity of Enzymes from the Amidohydrolase Superfamily.

Angewandte Chemie (International ed. in English)·2025
Same author

Activity Regulation of a Glutamine Amidotransferase Bienzyme Complex by Substrate-Induced Subunit Interface Expansion.

ACS catalysis·2025
Same author

A naturally occurring standalone TrpB enzyme provides insights into allosteric communication within tryptophan synthase.

Protein science : a publication of the Protein Society·2025
Same author

Photocontrolling the Enantioselectivity of a Phosphotriesterase via Incorporation of a Light-Responsive Unnatural Amino Acid.

JACS Au·2025

Related Experiment Video

Updated: Apr 4, 2026

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
05:08

Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

Published on: July 8, 2025

1.3K

Ancestral protein reconstruction: techniques and applications.

Rainer Merkl, Reinhard Sterner

    Biological Chemistry
    |September 10, 2015
    PubMed
    Summary

    Ancestral sequence reconstruction (ASR) enables studying ancient proteins and their functions. This powerful method reveals key amino acid residues and evolutionary insights not visible in modern proteins alone.

    Area of Science:

    • Biochemistry
    • Evolutionary Biology
    • Bioinformatics

    Background:

    • Ancestral sequence reconstruction (ASR) infers ancient protein sequences from extant ones.
    • ASR combined with experimental protein characterization addresses intractable biological questions.
    • Historical approaches in ASR identify key residues for protein function, which is challenging with extant proteins alone.

    Purpose of the Study:

    • To explore diverse applications of ancestral sequence reconstruction (ASR).
    • To highlight ASR's utility in studying ancient enzymes, protein pigments, and receptors.
    • To introduce the fundamental principles of ASR algorithms and current protocols.

    Main Methods:

    • Utilizing extant protein sequences to computationally reconstruct ancestral protein sequences.

    More Related Videos

    Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
    11:57

    Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

    Published on: April 21, 2016

    7.1K
    The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
    12:22

    The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

    Published on: January 22, 2013

    34.3K

    Related Experiment Videos

    Last Updated: Apr 4, 2026

    Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins
    05:08

    Application of I TASSER, trRosetta, UCSF Chimera, HADDOCK server, and HEX loria for De Novo and In Silico Design of Proteins

    Published on: July 8, 2025

    1.3K
    Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
    11:57

    Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions

    Published on: April 21, 2016

    7.1K
    The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
    12:22

    The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

    Published on: January 22, 2013

    34.3K
  • Integrating ancestral sequence reconstruction with experimental characterization of reconstructed proteins.
  • Applying ASR to investigate enzyme evolution, mutation longevity, and gene duplication impacts.

    • Main Results:

    • ASR successfully characterized ancient enzymes from the Paleoarchean era and their habitats.
    • Key amino acid residues responsible for protein pigment spectroscopic properties were identified.
    • Residues critical for steroid receptor binding specificity were elucidated through ASR.

    Conclusions:

    • ASR is a powerful tool for understanding protein evolution and function.
    • This approach provides unique insights into historical biological processes and molecular mechanisms.
    • Recent advancements offer state-of-the-art protocols for broad ASR applications.