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Does dexamethasone act in neuropeptides SP and CGRP in neurogenic inflammation of the skin? An experimental study
Jose Octavio Gonçalves de Freitas1, Paulo Rogério Quieregatto2, Bernardo Hochman2
1Universidade Federal de São Paulo, BR.
Purpose:
To investigate the neuropeptides substance P (SP) and calcitonin gene-related peptide (CGRP) after subcutaneous injection of dexamethasone prior to skin incision in rats.
Methods:
Twenty seven Wistar-EPM-1 rats were randomly divided into three groups. The sham group (SG) of rats was injected with 0.9 % saline. The second group (Dexa) was injected with 1.0 mg/kg dexamethasone, and the third group (Dexa+) was injected with 10.0 mg/kg dexamethasone. In all groups, the three subcutaneous injections were performed 30 minutes prior to the surgical skin incision and tissue collection. SP and CGRP (15 kDa pro-CGRP and 5 kDa CGRP) were quantified by Western Blotting.
Results:
No statistically significant differences (p>0.05) were found in pro-CGRP, CGRP and SP values in all three groups.
Conclusion:
The anti-inflammatory effect of dexamethasone did not occur when the substance P and calcitonin gene-related peptide levels were altered during the neurogenic inflammation process of skin wound healing in rats.
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