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Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy
Published on: September 8, 2023
Fluorescence polarization measurement system using a liquid crystal layer and an image sensor
Osamu Wakao1, Yusaku Fujii2, Masatoshi Maeki3
1Graduate School of Chemical Sciences and Engineering, Hokkaido University , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan.
Abstract:
The detection system which enables simultaneous fluorescence polarization (FP) measurement of multiple samples was proposed and proven by a proof-of-concept experiment on the viscosity dependence of FP of fluorescein sample in water-ethylene glycol solution and another experiment on the FP immunoassay of prostaglandin E2 sample. The measurement principle of FP is based on the synchronization between the orientation of the liquid crystal molecules and the sampling frequency of a CCD. This report is the first description of the simultaneous FP measurement of multiple samples. This system has a great potential for equipment miniaturization and price reduction as well as providing simultaneous FP measurement of multiple samples.
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