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Updated: Apr 4, 2026

Ammonia Fiber Expansion AFEX Pretreatment of Lignocellulosic Biomass
Published on: April 18, 2020
Enhancing saccharification of wheat straw by mixing enzymes from genetically-modified Trichoderma reesei and
Yanping Jiang1, Alexandra Vivas Duarte2,3, Joost van den Brink2,3
1Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China.
Objectives:
To increase the efficiency of enzymatic hydrolysis for plant biomass conversion into renewable biofuel and chemicals.
Results:
By overexpressing the point mutation A824 V transcriptional activator Xyr1 in Trichoderma reesei, carboxymethyl cellulase, cellobiosidase and β-D-glucosidase activities of the best mutant were increased from 1.8 IU/ml, 0.1 IU/ml and 0.05 IU/ml to 4.8 IU/ml, 0.4 IU/ml and 0.3 IU/ml, respectively. The sugar yield of wheat straw saccharification by combining enzymes from this mutant and the Aspergillus niger genetically modified strain ΔcreA/xlnR c/araR c was improved up to 7.5 mg/ml, a 229 % increase compared to the combination of wild type strains.
Conclusions:
Mixing enzymes from T. reesei and A. niger combined with the genetic modification of transcription factors is a promising strategy to increase saccharification efficiency.
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