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In situ patterned micro 3D liver constructs for parallel toxicology testing in a fluidic device.

Aleksander Skardal1, Mahesh Devarasetty, Shay Soker

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Health, Medical Center Boulevard, Winston-Salem, NC 27157, USA. Virginia Tech-Wake Forest University School of Biomedical Engineering and Sciences, Wake Forest Baptist Health, Medical Center Boulevard, Winston-Salem, NC 27157, USA. Comprehensive Cancer Center, Wake Forest Baptist Health, Medical Center Boulevard, Winston-Salem, NC 27157, USA.

Biofabrication
|September 11, 2015
PubMed
Summary

This study presents a simple, scalable microfluidic method for creating 3D liver tissue models for drug and toxicology testing. These engineered tissues accurately predict alcohol toxicity, showing reduced viability and function with increased exposure.

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Area of Science:

  • Biotechnology
  • Tissue Engineering
  • Microfluidics

Background:

  • 3D tissue models are crucial for drug and toxicology testing.
  • Current biofabrication methods are complex, costly, and difficult to scale.
  • There is a need for efficient and high-throughput tissue engineering approaches.

Purpose of the Study:

  • To develop a simple, scalable microfluidic strategy for creating 3D tissue constructs.
  • To create functional liver tissue models for toxicology applications.
  • To demonstrate the utility of these models in predicting alcohol-induced toxicity.

Main Methods:

  • Utilized a parallel microfluidic platform with UV-crosslinkable hydrogels.
  • Photopatterned liver cells within hydrogel channels to form in situ tissue constructs.
  • Cultured constructs for 7 days under recirculating media flow and assessed functionality.

Main Results:

  • Successfully fabricated stable and functional 3D liver tissue constructs in a microfluidic device.
  • Demonstrated that constructs mimic native tissue properties and extracellular matrix.
  • Showed decreased cell viability and altered urea/albumin secretion with increasing ethanol exposure, indicating toxicity.

Conclusions:

  • The developed microfluidic platform offers a simple, scalable, and high-throughput method for 3D tissue engineering.
  • These engineered liver tissues serve as a valuable tool for toxicology screening.
  • The model accurately reflects alcohol-induced cellular damage and functional impairment.