Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

111
Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
111
Evolution of Microbial Genome01:08

Evolution of Microbial Genome

48
Microbial genome evolution is a highly dynamic process shaped by continual gene gain and loss across species and strains. This genomic flexibility allows microorganisms to adapt rapidly to environmental pressures and interactions with other organisms. Central to understanding this diversity is the distinction between the core and pan genomes.The core genome comprises the genes shared by all sampled strains of a species, representing essential functions needed for fundamental cellular processes.
48
Stringent Response in E. coli01:23

Stringent Response in E. coli

469
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
469

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biofilm formation and associated biomechanical traits co-segregate with multidrug resistance in typhoidal Salmonella.

The Journal of antibiotics·2026
Same author

Antibacterial efficacy and adaptive proteomic strategies of antibiotic-resistant pathogens on nanostructured copper surfaces.

Nanomedicine (London, England)·2026
Same author

Protein expression and morphological adaptations of Campylobacter jejuni under prolonged cold stress in chicken juice.

Food microbiology·2026
Same author

Tracking the pandemic through molecular and sequencing tools: a story of SARS- CoV-2 over five years, lessons learned, and further directions.

BMC infectious diseases·2025
Same author

Quantitative proteomic and phenotypic responses of urinary pathogens to CuO/Cuâ‚‚O nanoparticles.

Nanomedicine (London, England)·2025
Same author

Association of leukocyte telomere length with anti-HBsAg antibody titer in Hepatitis-B recovered patients from the Pakistani population.

BMC infectious diseases·2025

Related Experiment Video

Updated: Apr 4, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K

Draft Genome Sequence of a vanA-Type Vancomycin-Resistant Reference Strain, Enterococcus faecium ATCC 51559.

Kidon Sung1, Saeed Khan2, Bernard Marasa2

  • 1Division of Microbiology, National Center for Toxicological Research (NCTR), U.S. Food and Drug Administration, Jefferson, Arkansas, USA kidon.sung@fda.hhs.gov.

Genome Announcements
|September 12, 2015
PubMed
Summary

We sequenced the genome of a high-level vancomycin-resistant Enterococcus faecium strain, ATCC 51559. This vanA genotype-positive strain is a key control for antimicrobial resistance studies.

More Related Videos

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.3K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.4K

Related Experiment Videos

Last Updated: Apr 4, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
09:44

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

3.1K
Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.3K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.4K

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Enterococcus faecium is a significant cause of hospital-acquired infections.
  • The emergence of vancomycin-resistant strains poses a major public health threat.
  • Multidrug-resistant pathogens require robust diagnostic and research tools.

Purpose of the Study:

  • To provide the draft genome sequence of a high-level vancomycin-resistant Enterococcus faecium strain.
  • To characterize the vanA genotype of the reference strain ATCC 51559.
  • To support clinical and laboratory studies involving vancomycin-resistant Enterococcus faecium.

Main Methods:

  • Whole-genome sequencing of Enterococcus faecium ATCC 51559.
  • Bioinformatic analysis for genome assembly and annotation.
  • Identification of the vanA gene conferring vancomycin resistance.

Main Results:

  • The draft genome sequence of Enterococcus faecium ATCC 51559 was successfully generated.
  • The presence of the vanA genotype was confirmed.
  • This strain serves as a validated vanA genotype-positive control.

Conclusions:

  • The genome sequence provides a valuable resource for understanding vancomycin resistance mechanisms in Enterococcus faecium.
  • The characterized strain ATCC 51559 is crucial for standardizing antimicrobial susceptibility testing and research.
  • Availability of this reference strain aids in the development of effective therapeutic strategies against resistant pathogens.