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Draft Genome Sequence of a vanA-Type Vancomycin-Resistant Reference Strain, Enterococcus faecium ATCC 51559

Kidon Sung1, Saeed Khan2, Bernard Marasa2

  • 1Division of Microbiology, National Center for Toxicological Research (NCTR), U.S. Food and Drug Administration, Jefferson, Arkansas, USA kidon.sung@fda.hhs.gov.

Genome Announcements
|September 12, 2015
PubMed

Insights

We sequenced the genome of a high-level vancomycin-resistant Enterococcus faecium strain, ATCC 51559. This vanA genotype-positive strain is a key control for antimicrobial resistance studies.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Enterococcus faecium is a significant cause of hospital-acquired infections.
  • The emergence of vancomycin-resistant strains poses a major public health threat.
  • Multidrug-resistant pathogens require robust diagnostic and research tools.

Purpose of the Study:

  • To provide the draft genome sequence of a high-level vancomycin-resistant Enterococcus faecium strain.
  • To characterize the vanA genotype of the reference strain ATCC 51559.
  • To support clinical and laboratory studies involving vancomycin-resistant Enterococcus faecium.

Main Methods:

  • Whole-genome sequencing of Enterococcus faecium ATCC 51559.
  • Bioinformatic analysis for genome assembly and annotation.
  • Identification of the vanA gene conferring vancomycin resistance.

Main Results:

  • The draft genome sequence of Enterococcus faecium ATCC 51559 was successfully generated.
  • The presence of the vanA genotype was confirmed.
  • This strain serves as a validated vanA genotype-positive control.

Conclusions:

  • The genome sequence provides a valuable resource for understanding vancomycin resistance mechanisms in Enterococcus faecium.
  • The characterized strain ATCC 51559 is crucial for standardizing antimicrobial susceptibility testing and research.
  • Availability of this reference strain aids in the development of effective therapeutic strategies against resistant pathogens.

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