Related Experiment Video
Updated: Apr 4, 2026
10:03
Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
Published on: January 20, 2014
10.2K
Pluripotent Conversion of Muscle Stem Cells Without Reprogramming Factors or Small Molecules
Bipasha Bose1,2, Sudheer Shenoy P3,4
1School of Biological Sciences, Nanyang Technological University, 60, Nanyang Drive, Singapore, 637551, Singapore. Bipasha.bose@gmail.com.
Stem Cell Reviews and Reports
|September 12, 2015
Summary
Muscle derived stem cells (MDSCs) from myostatin null mice were reprogrammed into pluripotent stem cells (CiPSCs) without genetic modification. This discovery offers a novel pathway for generating induced pluripotent stem cells from adult stem cells.
Area of Science:
- Stem cell biology
- Epigenetics
- Developmental biology
Background:
- Muscle derived stem cells (MDSCs) are multipotent adult stem cells with potential for differentiation.
- Myostatin is a key regulator of muscle growth and differentiation.
- Induced pluripotent stem cells (iPSCs) offer regenerative medicine potential but often require genetic manipulation.
Purpose of the Study:
- To investigate the potential of myostatin null MDSCs for reprogramming into pluripotent stem cells.
- To characterize the properties of these reprogrammed cells.
- To elucidate the underlying epigenetic mechanisms driving pluripotency.
Main Methods:
- Culturing MDSCs from myostatin null (Mstn (-/-)) and wild-type mice.
- Inducing pluripotency using embryonic stem cell media supplemented with Leukemia Inhibitory Factor (LIF).
- Analyzing cell markers (Oct4, Nanog, Sox2, SSEA-1), karyotype, differentiation potential (embryoid bodies, teratomas), and epigenetic modifications (BMP2 methylation).
Main Results:
- Mstn (-/-) MDSCs readily formed embryonic stem cell (ES) like colonies, termed culture-induced pluripotent stem cells (CiPSCs).
- CiPSCs expressed pluripotency markers, maintained a normal karyotype, and formed teratomas.
- CiPSCs differentiated into all three germ lineages.
- Hypermethylation and downregulation of BMP2 were observed in Mstn (-/-) MDSCs cultured in ES media, suggesting epigenetic reprogramming.
Conclusions:
- Myostatin deficiency epigenetically reprograms MDSCs into pluripotent stem cells under specific culture conditions.
- This method establishes ES-like cells from adult stem cells without reprogramming factors.
- The findings present a novel, non-genetic approach to generating induced pluripotent stem cells.
More Related Videos
Related Concept Videos
01:06Induced Pluripotent Stem Cells
6.3K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
6.3K
01:13Induced Pluripotent Stem Cells
28.6K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.6K
01:27Source And Potency Of Stem Cells
6.8K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
6.8K
01:21Satellite Stem Cells and Muscular Dystrophy
2.6K
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
2.6K
01:29Somatic to iPS Cell Reprogramming
2.9K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.9K
01:24Methods of Nuclear Reprogramming
2.3K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.3K