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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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Non-targeted Tracer Fate Detection.

Daniel Weindl1, André Wegner1, Karsten Hiller1

  • 1Luxembourg Centre for Systems Biomedicine, University of Luxembourg, Esch-Belval, Luxembourg.

Methods in Enzymology
|September 12, 2015
PubMed
Summary
This summary is machine-generated.

This study introduces the non-targeted tracer fate detection (NTFD) algorithm. NTFD automates the detection of isotopic enrichment, streamlining metabolic flux analysis and tracer studies.

Keywords:
(13)CCarbon-13Isotope labelingNTFDNon-targetedStable isotopesTracer fate

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Area of Science:

  • Metabolomics
  • Systems Biology
  • Biochemistry

Background:

  • Stable isotopes are crucial for tracing atoms in metabolism and quantifying metabolic fluxes.
  • Non-targeted stable isotope labeling offers powerful insights into metabolic pathways.
  • Manual detection of isotopic enrichment is laborious and time-consuming.

Purpose of the Study:

  • To develop an automated method for detecting isotopic enrichment in non-targeted metabolomics.
  • To introduce the non-targeted tracer fate detection (NTFD) algorithm.
  • To facilitate metabolic flux analysis and the identification of tracer-derived molecules.

Main Methods:

  • Development of the non-targeted tracer fate detection (NTFD) algorithm.
  • Automated detection and quantification of isotopic enrichment using mass isotopomer distributions (MIDs).
  • Description of the algorithmic background and practical considerations for the NTFD software package.

Main Results:

  • The NTFD algorithm automates the detection and quantification of isotopic enrichment.
  • NTFD enables tracing of functional groups and determination of MIDs for metabolic flux analysis.
  • The software package is freely available for broader application.

Conclusions:

  • NTFD significantly reduces the time and effort required for non-targeted stable isotope labeling analysis.
  • The algorithm provides a robust tool for advancing metabolome-wide studies.
  • NTFD has broad potential applications in various areas of biological research.