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Updated: Apr 4, 2026
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Published on: November 4, 2016
[Interleukin-37 inhibits the motility and migration of mouse splenic dendritic cells]
Yuechen Luo1, Fang Liu1, Wenwen Li1
1State Key Laboratory of Experimental Hematology, Institute of Hematology, Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, China.
Objective:
To investigate the effect of interleukin-37 (IL-37) on the motility and migration of mouse splenic dendritic cells (DCs).
Methods:
Magnetic bead cell sorting was used to isolate mouse splenic DCs, and flow cytometry was used to analyze the purity. DCs were visualized in real time by PerkinElmer laser confocal microscopy and analyzed by Volocity image analysis software. The alteration of chemokine (C motif) receptor 1 (XCR1) mRNA was revealed by microarray analysis and confirmed by real-time PCR and flow cytometry. Migration ability of DCs was detected by Transwell(TM) migration assay.
Results:
The purity of isolated DCs was over 95%. The treatment of IL-37 reduced the velocity of DCs mobility, decreased XCR1 expression and depressed the cell migration ability.
Conclusion:
IL-37 can inhibit the motility and migration of mouse splenic DCs.
