[Establishment and identification of Jurkat cell xenograft mouse models with over-expression of C-terminal Src kinase
Meihua Gao1, Yu Ma1, Bing Wang1
1Department of Immunology, Medical College, Qingdao University, Qingdao 266071, China.
Objective:
To establish xenograft mouse models of Jurkat T-leukemia cells over-expressing C-terminal Src kinase binding protein (CBP).
Methods:
The 5-week-old female BALB/c-nu mice were randomly divided into blank control group, normal Jurkat cell control group, empty virus-transfected Jurkat cell control group and CBP over-expression model group, 5 mice in each group. The mice were subcutaneously injected 1×10(7)/0.1 mL Jurkat cells in axillary area. The tumor tissues of mouse models were weighed and then subjected to HE staining to observe the pathological changes of tumor tissues. The proliferation of Jurkat cells in the peripheral blood of mice was detected by flow cytometry, and the interleukin 2 (IL-2) levels in mouse sera were determined with ELISA.
Results:
The volume of tumor tissues in the CBP over-expression model group was smaller than that in the control groups, so was the mass of tumor tissues. HE staining showed the proliferation of Jurkat cells in tumor tissues of the model group. The proliferation rate of Jurkat cells in the peripheral blood and IL-2 levels in the sera of the CBP over-expression model group were lower than those in the normal Jurkat cell control group and the empty virus-transfected Jurkat cell control group.
Conclusion:
The mouse models of Jurkat T-leukemia cells over-expressing CBP have been established successfully. Up-regulated CBP has inhibitory effects on the proliferation of Jurkat cells and IL-2 secretion.
