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Updated: Apr 4, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
[Preparation and application of polyclonal antibody against amelotin polypeptide]
Juanjuan Zhang1, Honghai Ji1, Yunxia Liu1
1Institute of Stomatology, School of Stomotalogy, Weifang Medical University, Weifang 261053, China.
Objective:
To prepare and identify the rabbit anti-mouse polyclonal antibody against amelotin polypeptide.
Methods:
The polypeptide was synthesized based on the bioinformatics analysis of amelotin, and coupled with keyhole limpet hemocyanin (KLH) for immunization. The amelotin polypeptide-KLH was injected into New Zealand rabbits to prepare the polyclonal antibody. ELISA technology was used to detect the titer of the antibody. The specificity of the polyclonal antibody was identified by Western blotting. The expression of amelotin in the submandibular tissue of mice was observed by immunohistochemistry.
Results:
ELISA showed that the titer of amelotin antibody was 1:1,000,000. Western blotting verified that the antibody had a high specificity. Immunohistochemistry indicated that amelotin was highly expressed in odontoprisis full-thick enamel of 3-day-old and 7-day-old mice, and also expressed in duct epithelial cytoplasm of submandibular glands of 7-day-old mice.
Conclusion:
The polyclonal antibody against amelotin polypeptide has been prepared successfully with high titer and high specificity.
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