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Analysis of CASP8 D302H Gene Variants in Patients with Primary Brain Tumors
Canan Cacina1, Sadrettin Pence1, Saime Turan1
1Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul, Turkey.
Background:
Alteration in cell-cycle control and apoptosis pathways play important roles in tumorigenesis. Caspase-8 (CASP8) is a member of the cysteine protease family, that is implicated in apoptosis regulation. The present study was designed to investigate the possible role of CASP8 D302H gene polymorphism in the tumor development.
Materials And Methods:
A total of 91 patients with brain tumors (including 39 meningioma and 52 glioma cases) and 114 healthy controls were included in the study. We investigated CASP8 D302H polymorphism by using polymorphism chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis.
Results:
The CASP8 D302H polymorphism genotypic frequencies were not statistically significantly different between meningioma cases and controls, with frequencies of GG, GC and CC genotypes of 71.2%, 19,2% and 9.6%; and 57.9%, 36.8% and 5.3%, respectively. The GG/CC genotypic frequencies were significantly increased in patients with glioma patients compared to controls (p=0.023) (χ(2)=5.149, odds ratio [OR]=1.27, 95% confidence interval [CI]=1.054-1.551). According to tumor characteristics, there were no statistically significant differences within the groups with astrocytic, oligoastrocytic tumors and oligodentriogliomas.
Conclusion:
D302H polymorphism of CASP8 gene may be associated with increased risk of glioma but larger study groups in different ethnic populations are needed to better elucidate the role of CASP8 gene polymorphism in the pathogenesis of primary brain tumors.
Insights
The CASP8 D302H gene polymorphism may increase glioma risk. Further research in diverse populations is needed to confirm its role in brain tumor development.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Cell-cycle control and apoptosis are crucial in tumorigenesis.
- Caspase-8 (CASP8) regulates apoptosis and is implicated in cancer development.
- The CASP8 D302H gene polymorphism's role in brain tumors requires investigation.
Purpose of the Study:
- To investigate the association between CASP8 D302H gene polymorphism and brain tumor risk.
- To analyze genotypic frequencies in meningioma and glioma patients compared to controls.
Main Methods:
- Study included 91 brain tumor patients (39 meningioma, 52 glioma) and 114 healthy controls.
- CASP8 D302H polymorphism was analyzed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
Main Results:
- No significant difference in CASP8 D302H genotypic frequencies was observed between meningioma patients and controls.
- A significant increase in GG/CC genotypic frequencies of CASP8 D302H was found in glioma patients compared to controls (p=0.023).
- No significant differences were found based on specific tumor subtypes (astrocytic, oligoastrocytic, oligodendroglioma).
Conclusions:
- The CASP8 D302H polymorphism may be linked to an elevated risk of glioma.
- Larger, multi-ethnic studies are necessary to fully understand the CASP8 gene's role in primary brain tumor pathogenesis.
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