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Updated: Apr 4, 2026

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
Published on: January 20, 2019
Study of the inflammatory response of immunocytes to polymerized porcine hemoglobin (pPolyHb)
Wenjin Zhu1, Fang Liu2, Jianguo Xin3
1a The First Affiliated Hospital of Xi'an Jiaotong University , Xi'an , P. R. China.
Abstract:
Polymerized porcine hemoglobin (pPolyHb), which was synthesized from chemically modified porcine hemoglobin, can carry and deliver oxygen to tissues in addition to restoring intravascular volume. Assessment of the inflammatory response generated in the host is a part of the overall safety evaluation of pPolyHb. In this study, we measured the levels of IL-1β, IL-10, and CD11b/CD18 in response to pPolyHb stimulation, both in vivo (in rats) and in vitro. Our results suggest that the levels of these indicators are not statistically changed by pPolyHb, indicating that pPolyHb is not immunotoxic to cells and animals in this respect.
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