Related Experiment Video

Updated: Apr 4, 2026

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants
07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

914

Twist1-mediated 4E-BP1 regulation through mTOR in non-small cell lung cancer

Tangfeng Lv1,2, Qian Wang1,3, Meghan Cromie1

  • 1Department of Environmental Toxicology, The Institute of Environmental and Human Health, Texas Tech University, Lubbock, Texas 79416, United States of America.

Oncotarget
|September 12, 2015
PubMed

Insights

Twist1 overexpression is linked to poor survival in non-small cell lung cancer (NSCLC). This study reveals Twist1 influences tumor growth and motility, correlating with p-4E-BP1, a key prognostic marker in NSCLC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Twist1 overexpression correlates with poor survival in non-small cell lung cancer (NSCLC).
  • The precise molecular mechanisms underlying Twist1's role in NSCLC tumorigenesis remain unclear.
  • Understanding Twist1's function is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the tumorigenic role of Twist1 in NSCLC.
  • To elucidate the molecular mechanisms associated with Twist1 in NSCLC.
  • To explore the correlation between Twist1 and p-4E-BP1 in NSCLC prognosis.

Main Methods:

  • Twist1 expression analysis in NSCLC patient samples and cell lines (H1650, H1975).
  • In vitro assays (wound closure, colony formation) using stable Twist1 knockdown (H1650shTw) and overexpression (H1975Over) cell lines.
  • In vivo tumor growth studies in mice.
  • Analysis of key molecular markers including 4E-BP1, p-4E-BP1, p-mTOR, and p53 gene/protein expression.
  • RT-PCR array analysis.

Main Results:

  • Twist1 was overexpressed in 34.7% of NSCLC patients, with higher expression linked to lower survival rates.
  • Twist1 manipulation affected cell motility, wound closure, and colony formation in vitro.
  • Tumor growth was significantly reduced in mice with Twist1 knockdown.
  • Twist1 inhibition decreased p-4E-BP1 and p-mTOR, while increasing p53 expression.
  • p-4E-BP1 was overexpressed in 24.0% of NSCLC patients, correlating with poor survival and Twist1 expression.

Conclusions:

  • Twist1 plays a significant tumorigenic role in NSCLC, impacting cell motility and growth.
  • Twist1 expression is correlated with p-4E-BP1, suggesting a combined prognostic value in NSCLC.
  • Twist1 inhibition may exert its effects by downregulating p-mTOR and upregulating p53, offering potential therapeutic targets.
Keywords:
Twist1epithelial-mesenchymal transitionnon-small cell lung cancerphosphorylated 4E-binding protein 1phosphorylated mammalian target of rapamycin

More Related Videos

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.4K
Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer06:51

Utilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer

Published on: July 21, 2018

18.9K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

1.7K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.4K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.0K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
4.0K