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Related Concept Videos

In Vitro Drug Dissolution: Alternative Methods01:17

In Vitro Drug Dissolution: Alternative Methods

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Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
347
In Vitro Drug Dissolution: Compendial Testing Models II01:09

In Vitro Drug Dissolution: Compendial Testing Models II

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Various dissolution methods are utilized to assess a drug’s dissolution rate, including the flow-through cell, paddle-over-disk, cylinder, and reciprocating disk methods.The flow-through cell apparatus (USP (United States Pharmacopeia) method 4) comprises a reservoir for the dissolution medium and a pump that propels the medium through the cell containing the test sample. This method is crucial for assessing modified-release dosage forms with minimally soluble active ingredients,...
572
In Vitro Drug Dissolution: Compendial Testing Models I01:13

In Vitro Drug Dissolution: Compendial Testing Models I

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Compendial dissolution methods are standardized procedures defined by pharmacopeias to evaluate the rate at which a drug dissolves in a specific medium. These methods ensure batch-to-batch consistency, enable quality control, and support the prediction of drug bioavailability. They are critical for both immediate and modified-release drug products.The apparatuses used for dissolution testing differ in their design and mechanical function, but all aim to simulate the physiological environment of...
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In Vitro Drug Release Testing: Overview, Development and Validation01:10

In Vitro Drug Release Testing: Overview, Development and Validation

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In vitro dissolution and drug release tests assess how quickly and how much of a drug is released from its dosage form into an aqueous medium under standardized laboratory conditions. These tests are essential tools in pharmaceutical development and quality assurance, offering insight into the drug's performance before clinical use.During formulation development, dissolution testing identifies incomplete or inconsistent drug release issues. It also supports decisions on selecting the optimal...
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Drug Dissolution: Requirements and Profile Comparison01:14

Drug Dissolution: Requirements and Profile Comparison

417
The acceptance criteria for dissolution profile data are anchored in Q values, representing the percentage of drug dissolved within a specified period. This assessment unfolds in three stages:First Stage: The test passes if all six drug dosage units are equal to or greater than Q plus 5%; otherwise, the sample proceeds to the second stage.Second Stage: The average of twelve units must be equal to or greater than Q, with no unit falling below Q - 15% to pass; if not, it progresses to the final...
417
Factors Affecting Dissolution: Particle Size and Effective Surface Area01:23

Factors Affecting Dissolution: Particle Size and Effective Surface Area

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Dissolution kinetics, an essential aspect of oral drug delivery, is significantly influenced by the drug's particle size. According to the Noyes-Whitney dissolution model, the dissolution rate correlates directly with the drug's surface area. The larger the surface area, the higher the drug's solubility in water, leading to a faster drug dissolution rate. Reducing particle size increases the effective surface area, enhancing the dissolution process. Micronization and nanosizing are...
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Related Experiment Video

Updated: Apr 4, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
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Miniaturized INtrinsic DISsolution Screening (MINDISS) assay for preformulation.

Jochem Alsenz1, Elisabeth Haenel1, Aline Anedda2

  • 1Roche Pharmaceutical Research & Early Development, Pre-Clinical CMC, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd., Grenzacherstrasse 124, Basel, Switzerland.

European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences
|September 12, 2015
PubMed
Summary

A new Miniaturized INtrinsic DISsolution Screening (MINDISS) assay enables rapid, parallel measurement of drug disk intrinsic dissolution rates (DIDR) using minimal compound and media. This assay is valuable for drug discovery and development.

Keywords:
Intrinsic dissolutionPharmaceutical profilingPolymorphsPreformulationRanking of drugsSaltsSmall scale

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Area of Science:

  • Pharmaceutical Sciences
  • Drug Delivery
  • Analytical Chemistry

Background:

  • Accurate measurement of intrinsic dissolution rates (DIDR) is crucial for drug development.
  • Traditional DIDR methods often require significant amounts of drug substance and dissolution media.
  • There is a need for miniaturized, high-throughput assays for early-stage drug screening.

Purpose of the Study:

  • To introduce and validate a novel Miniaturized INtrinsic DISsolution Screening (MINDISS) assay.
  • To demonstrate the utility of MINDISS for rapid, parallel determination of DIDR.
  • To showcase MINDISS's applicability in various stages of drug discovery and development.

Main Methods:

  • Preparation of compacted mini disks (2-5mg) in custom holders (3mm² surface area).
  • Immersion of disks in 0.35ml dissolution media within 96-well plates with defined time points and media transfer.
  • Quantification of drug concentration via Ultra Performance Liquid Chromatography (UPLC) and solid-state characterization using Raman spectroscopy.

Main Results:

  • MINDISS provides an easy-to-use tool for rapid, parallel DIDR determination.
  • The assay requires minimal amounts of compound and dissolution medium.
  • Results from MINDISS correlate well with established large-scale DIDR methods for marketed drugs.

Conclusions:

  • MINDISS is suitable for rank-ordering compounds by intrinsic dissolution in late discovery and early development.
  • The assay facilitates comparison of different polymorphic forms and salts.
  • MINDISS aids in screening dissolution media and characterizing intestinal release behavior by utilizing biorelevant media.