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Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...

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Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Fabrication and Operation of an Oxygen Insert for Adherent Cellular Cultures

Published on: January 6, 2010

A 3D-Printed Oxygen Control Insert for a 24-Well Plate.

Martin D Brennan¹, Megan L Rexius-Hall¹, David T Eddington¹

¹Dept of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States of America.

|September 12, 2015

Summary

This study introduces a novel 3D-printed microfluidic device with gas-permeable membranes for precise oxygen control in cell cultures. This innovation enables advanced cell culture studies by allowing tailored oxygen levels, enhancing gene expression analysis.

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Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Traditional microfluidic devices often use oxygen-impermeable materials, limiting their application in cell culture studies requiring oxygen control.
3D printing offers advantages in creating complex microfluidic geometries but has been constrained by material limitations for gas permeability.

Purpose of the Study:

To develop and validate a 3D-printed microfluidic device integrated with gas-permeable membranes for controlled oxygen environments in cell culture.
To demonstrate the device's capability in maintaining multiple, distinct oxygen conditions for parallel cell culture experiments.

Main Methods:

Fabrication of a 24-well microfluidic device using 3D printing technology.
Incorporation of Polydimethylsiloxane (PDMS) membranes to facilitate gas exchange and enable oxygen level control.
Culturing human lung adenocarcinoma cells under four different controlled oxygen conditions within the 3D-printed device.

Main Results:

The 3D-printed device successfully maintained six wells under each of the four distinct oxygen conditions.
Demonstrated enhanced transcription of the vascular endothelial growth factor A (VEGFA) gene in human lung adenocarcinoma cells with decreasing oxygen levels.
Successfully integrated 3D printing with gas-permeable membranes for advanced cell culture applications.

Conclusions:

The developed 3D-printed microfluidic device with integrated gas-permeable membranes is a viable tool for precise oxygen control in cell culture.
This technology enables complex cell culture studies and facilitates the investigation of oxygen-dependent biological processes, such as gene expression.
Represents a significant advancement in 3D-printed microfluidics for biological research and cell-based assays.