Plasmid-mediated chloramphenicol resistance in Staphylococcus hyicus
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S Schwarz1, M Cardoso, H Blobel
1Institut für Bakteriologie, Immunologie der Justus-Liebig Universität Giessen, FRG.
Journal of General Microbiology
|December 1, 1989
Abstract:
A small plasmid of 3.95 kb, encoding resistance to chloramphenicol (Cm) was detected in three of 33 Staphylococcus hyicus strains. The plasmid in each of the three strains was indistinguishable by Southern-blot hybridization and restriction enzyme analysis. It was shown by curing and by transformation to specify resistance to Cm. A preliminary restriction map of the plasmid, designated pSC2, is presented. Chloramphenicol acetyltransferase was demonstrated by enzyme assay and by SDS-PAGE of cell-free lysates of pSC2 transformants.